Matrix metalloproteases and their inhibitors are altered in torn rotator cuff tendons, but also in the macroscopically and histologically intact portion of those tendons

Alessandro Castagna, Eugenio Cesari, Raffaele Garofalo, Antonio Gigante, Marco Conti, Nikolaos Markopoulos, Nicola Maffulli

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated whether matrix metalloproteases and their inhibitors are involved in extracellular matrix remodelling and degradation of chronic rotator cuff tears. Tendon samples were harvested from 13 patients who underwent arthroscopic repair of a rotator cuff tear. Supraspinatus specimens were harvested en bloc from the arthroscopically intact middle portion of the tendon, more than 1 cm lateral to the torn edge, from the lateral edge of the tear, and from the superior margin of the macroscopically intact subscapularis tendon, used as control. The collagenases, the stromelysins, and the tissue inhibitors of metalloprotease arrays were analyzed blindly by multiplex sandwich ELISA in each specimen. Histological evidence of tendinopathy was present in all patients with a rotator cuff tear, but not in the macroscopically intact subscapularis tendon. There were significantly increased levels of MMP 1, MMP 2, MMP 3, TIMP-1, and TIMP-2 in all specimens examined, including the macroscopically intact portion of the supraspinatus tendon and the subscapularis (control specimens). The levels of specific matrix metalloproteases and their inhibitors are altered in torn rotator cuff tendons, but also in the macroscopically and histologically intact tendons. These changes extended medially to the site of tendon tear, and to other tendons.

Original languageEnglish
Pages (from-to)132-138
Number of pages7
JournalMuscles, Ligaments and Tendons Journal
Volume3
Issue number3
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Gene expression
  • Outcome
  • Rotator cuff
  • Surgery

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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