Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma

A. Leonardi, P. Brun, A. Di Stefano, L. Motterle, G. Abatangelo

Research output: Contribution to journalArticle

Abstract

Background: Allergic conditions in different organs share many similarities in their inflammatory response. Vernal keratoconjunctivitis (VKC), asthma and nasal polyps exhibit several similar, but site-specific mucosal structural changes. The aim of the study was to investigate whether matrix metalloproteases contribute to different tissue remodelling aspects in different organs. Methods: Mucosal biopsies were obtained from conjunctiva of healthy donors, tarsal conjunctiva of vernal patients, bronchi of non-asthmatic subjects, bronchi of mild stable asthmatic patients, nasal mucosa of non-allergic donors and nasal polyps of allergic patients. Distribution of metalloprotease-1, -3, -9, -13, tissue inhibitor of metalloproteases-1, collagens I and III and the presence of eosinophils and CD4+ cells were evaluated by immunohistochemistry. Results: Collagens were highly diffuse in the giant papillae of VKC and in nasal polyps, and yet less increased in the subepithelium of asthmatic patients. Immunostaining for metalloprotease-1, -3, -9 and -13 was significantly higher in VKC compared with normal conjunctiva. Metalloprotease-9 staining was higher in the stroma of polyps vs. normal nasal mucosa, and only metalloprotease-13 was significantly more expressed in asthmatic vs. non-asthmatic subjects. Metalloprotease-9 immunostaining was more intense in vernal compared with other tissues. In all pathological tissues, metalloprotease-9-positive staining was in association with eosinophils and CD4+ cells. Conclusions: Expression of metalloproteases may play an important role in inducing the structural changes seen in VKC, nasal polyps and asthma. Tissue remodelling and gelatinase immunoexpression was more dramatic in giant papillae of vernal patients compared with other tissue sites of chronic allergic inflammation.

Original languageEnglish
Pages (from-to)872-879
Number of pages8
JournalClinical and Experimental Allergy
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Allergic Conjunctivitis
Metalloproteases
Conjunctiva
Nasal Polyps
Nasal Mucosa
Bronchi
Eosinophils
Collagen
Tissue Donors
Staining and Labeling
Asthma and Nasal Polyps
Gelatinases
Polyps
Immunohistochemistry
Inflammation
Biopsy

Keywords

  • Collagen
  • MMP
  • Nasal polyps
  • Tissue remodelling
  • Vernal keratoconjunctivitis

ASJC Scopus subject areas

  • Immunology

Cite this

Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma. / Leonardi, A.; Brun, P.; Di Stefano, A.; Motterle, L.; Abatangelo, G.

In: Clinical and Experimental Allergy, Vol. 37, No. 6, 06.2007, p. 872-879.

Research output: Contribution to journalArticle

Leonardi, A. ; Brun, P. ; Di Stefano, A. ; Motterle, L. ; Abatangelo, G. / Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma. In: Clinical and Experimental Allergy. 2007 ; Vol. 37, No. 6. pp. 872-879.
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AU - Abatangelo, G.

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AB - Background: Allergic conditions in different organs share many similarities in their inflammatory response. Vernal keratoconjunctivitis (VKC), asthma and nasal polyps exhibit several similar, but site-specific mucosal structural changes. The aim of the study was to investigate whether matrix metalloproteases contribute to different tissue remodelling aspects in different organs. Methods: Mucosal biopsies were obtained from conjunctiva of healthy donors, tarsal conjunctiva of vernal patients, bronchi of non-asthmatic subjects, bronchi of mild stable asthmatic patients, nasal mucosa of non-allergic donors and nasal polyps of allergic patients. Distribution of metalloprotease-1, -3, -9, -13, tissue inhibitor of metalloproteases-1, collagens I and III and the presence of eosinophils and CD4+ cells were evaluated by immunohistochemistry. Results: Collagens were highly diffuse in the giant papillae of VKC and in nasal polyps, and yet less increased in the subepithelium of asthmatic patients. Immunostaining for metalloprotease-1, -3, -9 and -13 was significantly higher in VKC compared with normal conjunctiva. Metalloprotease-9 staining was higher in the stroma of polyps vs. normal nasal mucosa, and only metalloprotease-13 was significantly more expressed in asthmatic vs. non-asthmatic subjects. Metalloprotease-9 immunostaining was more intense in vernal compared with other tissues. In all pathological tissues, metalloprotease-9-positive staining was in association with eosinophils and CD4+ cells. Conclusions: Expression of metalloproteases may play an important role in inducing the structural changes seen in VKC, nasal polyps and asthma. Tissue remodelling and gelatinase immunoexpression was more dramatic in giant papillae of vernal patients compared with other tissue sites of chronic allergic inflammation.

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