Maturation of tissue engineered cartilage implanted in injured and osteoarthritic human knees

Anthony P. Hollander, Sally C. Dickinson, Trevor J. Sims, Paola Brun, Roberta Cortivo, Elisaveta Kon, Maurilio Marcacci, Stefano Zanasi, Anna Borrione, Claudio De Luca, Alessandra Pavesio, Carlo Soranzo, Giovanni Abatangelo

Research output: Contribution to journalArticlepeer-review


The regeneration of damaged organs requires that engineered tissues mature when implanted at sites of injury or disease. We have used new analytic techniques to determine the extent of tissue regeneration after treatment of knee injury patients with a novel cartilage tissue engineering therapy and the effect of pre-existing osteoarthritis on the regeneration process. We treated 23 patients, with a mean age of 35.6 years, presenting with knee articular cartilage defects 1.5 cm2 to 11.25 cm2 (mean, 5.0 cm 2) in area. Nine of the patients had X-ray evidence of osteoarthritis. Chondrocytes were isolated from healthy cartilage removed at arthroscopy. The cells were cultured for 14 days, seeded onto esterified hyaluronic acid scaffolds (Hyalograft® C), and grown for a further 14 days before implantation. A second-look biopsy was taken from each patient after 6 to 30 months (mean, 16 months). After standard histological analysis, uncut tissue was further analyzed using a newly developed biochemical protocol involving digestion with trypsin and specific, quantitative assays for type II collagen, type I collagen, and proteoglycan, as well as mature and immature collagen crosslinks. Cartilage regeneration was observed as early as 11 months after implantation and in 10 out of 23 patients. Tissue regeneration was found even when implants were placed in joints that had already progressed to osteoarthrosis. Cartilage injuries can be effectively repaired using tissue engineering, and osteoarthritis does not inhibit the regeneration process.

Original languageEnglish
Pages (from-to)1787-1798
Number of pages12
JournalTissue Engineering
Issue number7
Publication statusPublished - Jul 2006

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

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