Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures

Claudia Winzler, Patrizia Rovere, Maria Rescigno, Francesca Granucci, Giuseppe Penna, Luciano Adorini, Valerie S. Zimmermann, Jean Davoust, Paola Ricciardi-Castagnoli

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Abstract

The signals controlling the checkpoints of dendritic cells (DC) maturation and the correlation between phenotypical and functional maturational stages were investigated in a defined model system of growth factor-dependent immature mouse DC. Three sequential stages of DC maturation (immature, mature, and apoptotic) were defined and characterized. Immature DC (stage 1) had low expression of costimulatory molecules, highly organized cytoskeleton focal adhesion plaques, and slow motility; accordingly, they were very efficient in antigen uptake and processing of soluble proteins. Further, at this stage most of major histocompatibility complex class II molecules were within cytoplasmic compartments consistent with a poor allostimulatory capacity. Bacteria or cytokines were very efficient in inducing progression from stage 1 towards stage 2 (mature). Morphological changes were observed by confocal analysis including depolymerization of F- actin and loss of vinculin containing adhesive structures which correlates with acquisition of high motility. Antigen uptake and presentation of native protein antigen was reduced. In contrast, presentation of immunogenic peptides and allostimulatory activity became very efficient and secretion of IL-12 p75 was detectable after antigen presentation. This functional DC maturation ended by apoptotic cell death, and no reversion to the immature phenotype was observed.

Original languageEnglish
Pages (from-to)317-328
Number of pages12
JournalJournal of Experimental Medicine
Volume185
Issue number2
DOIs
Publication statusPublished - Jan 20 1997

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Dendritic Cells
Intercellular Signaling Peptides and Proteins
Antigen Presentation
Focal Adhesions
Vinculin
Interleukin-12
Major Histocompatibility Complex
Cytoskeleton
Adhesives
Actins
Proteins
Cell Death
Cytokines
Bacteria
Phenotype
Antigens
Peptides

ASJC Scopus subject areas

  • Immunology

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Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures. / Winzler, Claudia; Rovere, Patrizia; Rescigno, Maria; Granucci, Francesca; Penna, Giuseppe; Adorini, Luciano; Zimmermann, Valerie S.; Davoust, Jean; Ricciardi-Castagnoli, Paola.

In: Journal of Experimental Medicine, Vol. 185, No. 2, 20.01.1997, p. 317-328.

Research output: Contribution to journalArticle

Winzler, C, Rovere, P, Rescigno, M, Granucci, F, Penna, G, Adorini, L, Zimmermann, VS, Davoust, J & Ricciardi-Castagnoli, P 1997, 'Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures', Journal of Experimental Medicine, vol. 185, no. 2, pp. 317-328. https://doi.org/10.1084/jem.185.2.317
Winzler, Claudia ; Rovere, Patrizia ; Rescigno, Maria ; Granucci, Francesca ; Penna, Giuseppe ; Adorini, Luciano ; Zimmermann, Valerie S. ; Davoust, Jean ; Ricciardi-Castagnoli, Paola. / Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures. In: Journal of Experimental Medicine. 1997 ; Vol. 185, No. 2. pp. 317-328.
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