Matured human monocyte-derived dendritic cells (MoDCs) induce expansion of CD4+CD25+FOXP3+ T cells lacking regulatory properties

Andrea Merlo, Elda Tagliabue, Sylvie Mènard, Andrea Balsari

Research output: Contribution to journalArticlepeer-review

Abstract

Naturally occurring CD4+CD25+ T regulatory cells (nTregs) play a key role as suppressors in immune mechanisms that protect against self-destruction. The forkhead box p3 transcription factor (FOXP3) has a central role in the development of nTregs. We show here that co-culture of naïve T cells with flagellin-exposed monocyte-derived dendritic cells (MoDCs) generates CD4+CD25+FOXP3+ T cells that transiently express FOXP3 together with CD25 but do not suppress proliferation of CD4+CD25- T cells. Moreover, purified CD4+CD25+FOXP3+ T cells reveal a different proliferation and cytokine production profile from that of nTregs. These data indicate that in the presence of ongoing immune responses a T cell antigenic phenotype superimposable of that of nTregs does not necessarily predict suppressive function and that FOXP3 in humans is not sufficient for development and function of regulatory T cells.

Original languageEnglish
Pages (from-to)106-113
Number of pages8
JournalImmunology Letters
Volume117
Issue number1
DOIs
Publication statusPublished - Apr 15 2008

Keywords

  • Dendritic cells
  • Flagellin
  • FOXP3
  • Regulatory T cells
  • Toll-like receptor ligands

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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