Max and c-Myc/Max DNA-binding activities in cell extracts

T. D. Littlewood, B. Amati, H. Land, G. I. Evan

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

We have examined the interactions and DNA-binding activities of the c-Myc oncoprotein and its partner Max. In cell extracts virtually all c-Myc molecules are associated with Max in heterodimeric complexes. Moreover, DNA-binding studies with in vitro-translated protein and cell extracts show that both Max alone and c-Myc/Max bind the same DNA sequence. Conversely, c-Myc is unable to bind this sequence in the absence of Max. These findings suggest that c-Myc may function via obligate complex formation with Max.

Original languageEnglish
Pages (from-to)1783-1792
Number of pages10
JournalOncogene
Volume7
Issue number9
Publication statusPublished - 1992

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Cell Extracts
Oncogene Proteins
DNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Littlewood, T. D., Amati, B., Land, H., & Evan, G. I. (1992). Max and c-Myc/Max DNA-binding activities in cell extracts. Oncogene, 7(9), 1783-1792.

Max and c-Myc/Max DNA-binding activities in cell extracts. / Littlewood, T. D.; Amati, B.; Land, H.; Evan, G. I.

In: Oncogene, Vol. 7, No. 9, 1992, p. 1783-1792.

Research output: Contribution to journalArticle

Littlewood, TD, Amati, B, Land, H & Evan, GI 1992, 'Max and c-Myc/Max DNA-binding activities in cell extracts', Oncogene, vol. 7, no. 9, pp. 1783-1792.
Littlewood TD, Amati B, Land H, Evan GI. Max and c-Myc/Max DNA-binding activities in cell extracts. Oncogene. 1992;7(9):1783-1792.
Littlewood, T. D. ; Amati, B. ; Land, H. ; Evan, G. I. / Max and c-Myc/Max DNA-binding activities in cell extracts. In: Oncogene. 1992 ; Vol. 7, No. 9. pp. 1783-1792.
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