(+)-MCPG induces PKCε translocation in cortical synaptosomes through a PLD-coupled mGluR

Lucia Pastorino, F. Colciaghi, F. Gardoni, S. Albani-Torregrossa, D. E. Pellegrini-Giampietro, F. Moroni, P. N E De Graan, F. Cattabeni, M. Di Luca

Research output: Contribution to journalArticle

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Abstract

We have tested whether different agonists of metabotropic glutamate receptors could induce translocation of selective protein kinase C isozymes in nerve terminals. In rat cortical synaptosomes 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD; 100 μM) induced an increase in translocation to 124.6 ± 5.7% of basal unstimulated conditions of the Ca++-independent protein kinase Cε, but not of the Ca++-dependent isozyme β. This effect was counteracted by 1-aminoindan-1,5-dicarboxylic acid (100 μM), an antagonist of metabotropic glutamate receptor 1. On the other hand, (+)-α-methyl-4-carboxyphenylglycine [(+)-MCPG], an antagonist of metabotropic glutamate receptors group I and II, did not antagonize the effect of 1S,3R-ACPD, and per se induced a translocation of protein kinase Cε of 164 ± 17.7% of basal unstimulated conditions. Because the (+)-MCPG induction of protein kinase Cε translocation was not antagonized by 1-aminoindan-1,5-dicarboxylic acid, it is suggested that 1S,3R-ACPD and (+)-MCPG activate this signal transduction pathway through distinct membrane receptors. Indeed (2-[2″-carboxy-3'-phenylcyclopropyl]glycine)-13 (300 nM), a new compound known to antagonize metabotropic glutamate receptors coupled to phospholipase D, was able to antagonize protein kinase Cε translocation induced by (+)-MCPG. Moreover (+)-MCPG directly induced phospholipase D activity, measured as [3H]phosphoethanol production in cortical synaptosomes. These data suggest that in cortical nerve terminals (i) distinct metabotropic glutamate receptors, coupled to different signal transduction pathways, are present, (ii) (+)-MCPG is able to induce protein kinase Cε translocation, and that (iii) a metabotropic glutamate receptor associated to phospholipase D might influence translocation of protein kinase C in a calcium-independent manner.

Original languageEnglish
Pages (from-to)1310-1318
Number of pages9
JournalEuropean Journal of Neuroscience
Volume12
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

Synaptosomes
Protein Kinase C
Metabotropic Glutamate Receptors
Phospholipase D
Isoenzymes
Signal Transduction
1-dodecylpyridoxal
Glycine
Calcium
Membranes
1-amino-1,3-dicarboxycyclopentane

Keywords

  • Cortex
  • Nerve terminal
  • Rat
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pastorino, L., Colciaghi, F., Gardoni, F., Albani-Torregrossa, S., Pellegrini-Giampietro, D. E., Moroni, F., ... Di Luca, M. (2000). (+)-MCPG induces PKCε translocation in cortical synaptosomes through a PLD-coupled mGluR. European Journal of Neuroscience, 12(4), 1310-1318. https://doi.org/10.1046/j.1460-9568.2000.00023.x

(+)-MCPG induces PKCε translocation in cortical synaptosomes through a PLD-coupled mGluR. / Pastorino, Lucia; Colciaghi, F.; Gardoni, F.; Albani-Torregrossa, S.; Pellegrini-Giampietro, D. E.; Moroni, F.; De Graan, P. N E; Cattabeni, F.; Di Luca, M.

In: European Journal of Neuroscience, Vol. 12, No. 4, 2000, p. 1310-1318.

Research output: Contribution to journalArticle

Pastorino, L, Colciaghi, F, Gardoni, F, Albani-Torregrossa, S, Pellegrini-Giampietro, DE, Moroni, F, De Graan, PNE, Cattabeni, F & Di Luca, M 2000, '(+)-MCPG induces PKCε translocation in cortical synaptosomes through a PLD-coupled mGluR', European Journal of Neuroscience, vol. 12, no. 4, pp. 1310-1318. https://doi.org/10.1046/j.1460-9568.2000.00023.x
Pastorino L, Colciaghi F, Gardoni F, Albani-Torregrossa S, Pellegrini-Giampietro DE, Moroni F et al. (+)-MCPG induces PKCε translocation in cortical synaptosomes through a PLD-coupled mGluR. European Journal of Neuroscience. 2000;12(4):1310-1318. https://doi.org/10.1046/j.1460-9568.2000.00023.x
Pastorino, Lucia ; Colciaghi, F. ; Gardoni, F. ; Albani-Torregrossa, S. ; Pellegrini-Giampietro, D. E. ; Moroni, F. ; De Graan, P. N E ; Cattabeni, F. ; Di Luca, M. / (+)-MCPG induces PKCε translocation in cortical synaptosomes through a PLD-coupled mGluR. In: European Journal of Neuroscience. 2000 ; Vol. 12, No. 4. pp. 1310-1318.
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AU - Pastorino, Lucia

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AU - Gardoni, F.

AU - Albani-Torregrossa, S.

AU - Pellegrini-Giampietro, D. E.

AU - Moroni, F.

AU - De Graan, P. N E

AU - Cattabeni, F.

AU - Di Luca, M.

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N2 - We have tested whether different agonists of metabotropic glutamate receptors could induce translocation of selective protein kinase C isozymes in nerve terminals. In rat cortical synaptosomes 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD; 100 μM) induced an increase in translocation to 124.6 ± 5.7% of basal unstimulated conditions of the Ca++-independent protein kinase Cε, but not of the Ca++-dependent isozyme β. This effect was counteracted by 1-aminoindan-1,5-dicarboxylic acid (100 μM), an antagonist of metabotropic glutamate receptor 1. On the other hand, (+)-α-methyl-4-carboxyphenylglycine [(+)-MCPG], an antagonist of metabotropic glutamate receptors group I and II, did not antagonize the effect of 1S,3R-ACPD, and per se induced a translocation of protein kinase Cε of 164 ± 17.7% of basal unstimulated conditions. Because the (+)-MCPG induction of protein kinase Cε translocation was not antagonized by 1-aminoindan-1,5-dicarboxylic acid, it is suggested that 1S,3R-ACPD and (+)-MCPG activate this signal transduction pathway through distinct membrane receptors. Indeed (2-[2″-carboxy-3'-phenylcyclopropyl]glycine)-13 (300 nM), a new compound known to antagonize metabotropic glutamate receptors coupled to phospholipase D, was able to antagonize protein kinase Cε translocation induced by (+)-MCPG. Moreover (+)-MCPG directly induced phospholipase D activity, measured as [3H]phosphoethanol production in cortical synaptosomes. These data suggest that in cortical nerve terminals (i) distinct metabotropic glutamate receptors, coupled to different signal transduction pathways, are present, (ii) (+)-MCPG is able to induce protein kinase Cε translocation, and that (iii) a metabotropic glutamate receptor associated to phospholipase D might influence translocation of protein kinase C in a calcium-independent manner.

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