Measurement and significance of antibodies against GM1 ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.

Donald M. Marcus, Norman Latov, Bart P. Hsi, Baiba K. Gillard, Jack Antel, Stanley Appel, Lanfranco Callegaro, Genevieve Daune, Baiba Gillard, Norman Latoy, Donald Marcus, Eduardo Nobile-Orazio, Alan Pestronk, Jacques Portoukalian, Richard Quarles, Edgar Salazar-Grueso, Larry Schneider, Michael Shy, Andreas Steck

Research output: Contribution to journalArticlepeer-review

Abstract

Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1 antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalJournal of Neuroimmunology
Volume25
Issue number2-3
DOIs
Publication statusPublished - 1989

Keywords

  • Anti-ganglioside
  • Enzyme-linked immunosorbent assay
  • Neuropathy

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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