Measurement of alkaline phosphatase activity in serum with N-methyl-D-glucamine as a buffer: Evaluation of the method for routine use

C. Franzini, G. Cattozzo, F. Ceriotti, C. A. Ferrero, P. Berti, P. P. Bianchi Bonvicini, G. Ceriotti, R. Deledda, D. Fenili, L. Morosini, R. Pagni, B. Prandini, A. Vernocchi

Research output: Contribution to journalArticle

Abstract

Some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using N-methyl-D-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. The evaluated characteristics included: the temperature-dependence of the pH of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the within-run and the between laboratory imprecision; the correlation with an alternative routine method. Also, sex- and age-related reference values were produced, based on 2968 values from selected reference sample groups in 7 laboratories. In general, the results demonstrate the robustness of the method, its adaptability to a variety of mechanized analysers, and hence its feasibility as a routine measurement method.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalEuropean Journal of Clinical Chemistry and Clinical Biochemistry
Volume29
Issue number11
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Franzini, C., Cattozzo, G., Ceriotti, F., Ferrero, C. A., Berti, P., Bianchi Bonvicini, P. P., Ceriotti, G., Deledda, R., Fenili, D., Morosini, L., Pagni, R., Prandini, B., & Vernocchi, A. (1991). Measurement of alkaline phosphatase activity in serum with N-methyl-D-glucamine as a buffer: Evaluation of the method for routine use. European Journal of Clinical Chemistry and Clinical Biochemistry, 29(11), 759-765.