Measurement of Alkaline Phosphatase Activity in Serum with N-Methyl-D-Glucamine as a Buffer

Evaluation of the Method for Routine Use

Carlo Franzini, Giampaolo Cattozzo, Ferruccio Ceriotti, Carlo A. Ferrero, P. Berti, P. Bianchi, P. Bonvicini, G. Ceriotti, R. Deledda, D. Fenili, L. Morosini, R. Pagni, B. Prandini, A. Vernocchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using N-methyl-D-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. The evaluated characteristics included: the temperature-dependence of the pH of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the within-run and the between laboratory imprecision; the correlation with an alternative routine method. Also, sex- and age-related reference values were produced, based on 2968 values from selected reference sample groups in 7 laboratories. In general, the results demonstrate the robustness of the method, its adaptability to a variety of mechanized analysers, and hence its feasibility as a routine measurement method.

Original languageEnglish
Pages (from-to)759-766
Number of pages8
JournalClinical Chemistry and Laboratory Medicine
Volume29
Issue number11
DOIs
Publication statusPublished - 1991

Fingerprint

Alkaline Phosphatase
Buffers
Starters
Serum
Reference Values
Magnesium
Zinc
Volume fraction
Ions
Substrates
Temperature

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Measurement of Alkaline Phosphatase Activity in Serum with N-Methyl-D-Glucamine as a Buffer : Evaluation of the Method for Routine Use. / Franzini, Carlo; Cattozzo, Giampaolo; Ceriotti, Ferruccio; Ferrero, Carlo A.; Berti, P.; Bianchi, P.; Bonvicini, P.; Ceriotti, G.; Deledda, R.; Fenili, D.; Morosini, L.; Pagni, R.; Prandini, B.; Vernocchi, A.

In: Clinical Chemistry and Laboratory Medicine, Vol. 29, No. 11, 1991, p. 759-766.

Research output: Contribution to journalArticle

Franzini, C, Cattozzo, G, Ceriotti, F, Ferrero, CA, Berti, P, Bianchi, P, Bonvicini, P, Ceriotti, G, Deledda, R, Fenili, D, Morosini, L, Pagni, R, Prandini, B & Vernocchi, A 1991, 'Measurement of Alkaline Phosphatase Activity in Serum with N-Methyl-D-Glucamine as a Buffer: Evaluation of the Method for Routine Use', Clinical Chemistry and Laboratory Medicine, vol. 29, no. 11, pp. 759-766. https://doi.org/10.1515/cclm.1991.29.11.759
Franzini, Carlo ; Cattozzo, Giampaolo ; Ceriotti, Ferruccio ; Ferrero, Carlo A. ; Berti, P. ; Bianchi, P. ; Bonvicini, P. ; Ceriotti, G. ; Deledda, R. ; Fenili, D. ; Morosini, L. ; Pagni, R. ; Prandini, B. ; Vernocchi, A. / Measurement of Alkaline Phosphatase Activity in Serum with N-Methyl-D-Glucamine as a Buffer : Evaluation of the Method for Routine Use. In: Clinical Chemistry and Laboratory Medicine. 1991 ; Vol. 29, No. 11. pp. 759-766.
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