Measurement of caspase activity: from cell populations to individual cells

Gabriela Paroni, Claudio Brancolini

Research output: Contribution to journalArticle

Abstract

Caspases are critical regulators of the apoptotic program, responsible for the harmonic dismantling of the cell. Cell death can occur by way of different options (necroptosis, necrosis, extreme autophagy) but once caspases are fully engaged it will take the apoptotic route. Hence, in general, caspase activity is inversely proportional to cell viability. Caspase activation can be measured by means of different techniques. Here, we describe three different methodologies to measure/observe caspase activation. Two approaches are recommended for studies on the whole cell population, whereas the third one is designed for visualizing caspase activation in single live cells.

Original languageEnglish
Pages (from-to)65-79
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume740
DOIs
Publication statusPublished - 2011

Keywords

  • Caspases/metabolism
  • Cells/enzymology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Activation
  • Fluorometry
  • Genes, Reporter
  • Humans
  • Immunoblotting
  • Membrane Potential, Mitochondrial
  • Single-Cell Analysis/methods
  • Time-Lapse Imaging

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