Measurement of cyclosporine in plasma of cardiac allograft recipients by fluorescence polarization immunoassay

R. Rondanelli, M. B. Regazzi, L. Gastaldi, P. Legnazzi, P. Abelli

Research output: Contribution to journalArticle

Abstract

The Abbott TDx fluorescence polarization immunoassay (FPIA) procedure for measuring cyclosporine A (CsA) was evaluated and compared with the Sandoz polyclonal radioimmunoassay (CsA RIA kit) method. This drug assay was evaluated for precision, calibration, stability, and accuracy. Within-run precision studies utilizing 25 replicate analyses of the three control preparations (containing CsA in the 60-800 ng/ml range) resulted in coefficients of variation (CV) ranging from 1.0 to 9.1%. The CVs of between-run precision determined by assaying the same control drug levels for five consecutive working days ranged from 3.9 to 4.6%. Calibration curve stability was assessed by examining the drift in control values over a 2-week period. Maximum plasma ranged from 82.6 to 108.2%. Four hundred plasma samples were obtained from 30 heart-transplant patients during the first 6 months of CsA therapy and each sample was analyzed simultaneously by TDx and RIA. Linear regression analysis of the results obtained for each patient (x = RIA, y = FPIA) revealed the following mean values: r = 0.87, (CV = 13.7%), slope = 1.47 (CV = 39.2%). Moreover, the concentration of CsA was determined in 35 patient samples both by TDx and high-performance liquid chromatography (HPLC). FPIA results up to 12 times higher than HPLC results have been noted.

Original languageEnglish
Pages (from-to)182-186
Number of pages5
JournalTherapeutic Drug Monitoring
Volume12
Issue number2
Publication statusPublished - 1990

Keywords

  • Cyclosporine A
  • Fluorescence polarization immunoassay
  • High-performance liquid chromatography
  • Radioimmunoassay

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Toxicology

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  • Cite this

    Rondanelli, R., Regazzi, M. B., Gastaldi, L., Legnazzi, P., & Abelli, P. (1990). Measurement of cyclosporine in plasma of cardiac allograft recipients by fluorescence polarization immunoassay. Therapeutic Drug Monitoring, 12(2), 182-186.