Measurement of erythrocyte acetylcholinesterase and plasma cholinesterase activity by a differential pH technique

L. Barenghi, F. Ceriotti, M. Luzzana, M. Ripamonti, A. Mosca, P. A. Bonini

Research output: Contribution to journalArticle

Abstract

We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are simplicity, high reproducibility, no need for pre-treatment of samples, automatic correction of sample blanks, and speed and direct measurement of enzymatic reaction. The proposed methods are linear up to 7400 U/L at 30°C and correlate well with the manual spectrophotometric method of Ellman for plasma cholinesterase and for washed erythrocytes. We adapted the same technique for the determination of erythrocyte cholinesterase using whole blood as sample and quinidine sulphate as inhibitor of pseudocholinesterase.

Original languageEnglish
Pages (from-to)538-545
Number of pages8
JournalAnnals of Clinical Biochemistry
Volume23
Issue number5
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Barenghi, L., Ceriotti, F., Luzzana, M., Ripamonti, M., Mosca, A., & Bonini, P. A. (1986). Measurement of erythrocyte acetylcholinesterase and plasma cholinesterase activity by a differential pH technique. Annals of Clinical Biochemistry, 23(5), 538-545.