Measurement of glucose in plasma by a differential pH technique

M. Luzzana, G. Dossi, A. Mosca, A. Granelli, D. Berger, E. Rovida, M. Ripamonti, A. Musetti, L. Rossi-Bernardi

Research output: Contribution to journalArticle


A new automatic apparatus based on the differential measurement of pH between two solutions has been developed. Two 25-μL (internal volume) glass capillary electrodes are used to measure the results of automated (under microcomputer control) chemical reactions that lead to the liberation or the uptake of hydrogen ions. The sensitivity of the differential pH measurements is better than ±0.0001 pH unit, and the change in H + concentration that can be detected by such an apparatus is 1 μmol/L for plasma and 3 μmol/L for whole blood. The technique has been applied to the measurement of glucose in plasma, giving results in agreement with the specifications of the Food and Drug Administration reference method for quantitative determination of glucose (hexokinase/glucose-6-phosphate dehydrogenase method).

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalClinical Chemistry
Issue number1
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Clinical Biochemistry

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  • Cite this

    Luzzana, M., Dossi, G., Mosca, A., Granelli, A., Berger, D., Rovida, E., Ripamonti, M., Musetti, A., & Rossi-Bernardi, L. (1983). Measurement of glucose in plasma by a differential pH technique. Clinical Chemistry, 29(1), 80-85.