Measurement of lipase activity by a differential pH technique

F. Ceriotti, P. A. Bonini, M. Murone, L. Barenghi, M. Luzzana, A. Mosca, M. Ripamonti, L. Rossi-Bernardi

Research output: Contribution to journalArticle

Abstract

This is a new electrochemical method for determination of lipase activity in biological fluids, including serum, plasma, and duodenal juice. Advantages of turbidimetric methods - short reaction time, and small sample and reagent volumes - are combined with those of titrimetric methods: measurement of absolute activity (i.e., no standardization required), saturated substrate conditions, and direct measurement of reaction products. The proposed method is easy, inexpensive, and takes only 3 min. Precision is good: CV = 3.74% within day and 7.3% between days at the clinical-decision concentration, V = 1.86% within day and 4.65% between days for above-normal lipase activities. The standard curve is linear up to 4500 U/L. Results (y) correlate well with those by turbidimetry (x); y = 0.9287x - 65.3 (r = 0.9719). Reference values are between 0 and 130 U/L.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalClinical Chemistry
Volume31
Issue number2
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Ceriotti, F., Bonini, P. A., Murone, M., Barenghi, L., Luzzana, M., Mosca, A., Ripamonti, M., & Rossi-Bernardi, L. (1985). Measurement of lipase activity by a differential pH technique. Clinical Chemistry, 31(2), 257-260.