Abstract
This is a new electrochemical method for determination of lipase activity in biological fluids, including serum, plasma, and duodenal juice. Advantages of turbidimetric methods - short reaction time, and small sample and reagent volumes - are combined with those of titrimetric methods: measurement of absolute activity (i.e., no standardization required), saturated substrate conditions, and direct measurement of reaction products. The proposed method is easy, inexpensive, and takes only 3 min. Precision is good: CV = 3.74% within day and 7.3% between days at the clinical-decision concentration, V = 1.86% within day and 4.65% between days for above-normal lipase activities. The standard curve is linear up to 4500 U/L. Results (y) correlate well with those by turbidimetry (x); y = 0.9287x - 65.3 (r = 0.9719). Reference values are between 0 and 130 U/L.
Original language | English |
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Pages (from-to) | 257-260 |
Number of pages | 4 |
Journal | Clinical Chemistry |
Volume | 31 |
Issue number | 2 |
Publication status | Published - 1985 |
ASJC Scopus subject areas
- Clinical Biochemistry