Measurement of phagocytosis utilizing [14C]cyanate-labelled human red cells and monocytes

F. Bussolino, F. Turrini, P. Arese

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Human red cells were labelled with [14C]cyanate, non-oxidant, permeant reagent that binds irreversibly to amino groups in proteins. Cyanate did not modify GSH levels, nor glucose-6-phosphate dehydrogenase (G6PD) activity when added at the labelling concentration i.e. c. 0.5 mM. Phagocytes were human monocytes, isolated and plated on 16 mm diameter plastic wells. Each well was plated with 40-70x103 cells. Monocytes were quantified by DNA assay with the DNA intercalating fluorescent compound Hoechst 33258. The basal phagocytic rate of normal red cells by monocytes was 0.34±0.21 red cell per monocyte. Treatment of normal red cells with 20 μM diamide, or 50-100 μM chromate enhanced this rate 10-15-fold. 10μM diamide or chromate were sufficient to stimulate phagocytosis of G6PD-deficient (Mediterranean variant) red cells.

Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalBritish Journal of Haematology
Volume66
Issue number2
Publication statusPublished - 1987

Fingerprint

Cyanates
Phagocytosis
Monocytes
Diamide
Chromates
Glucosephosphate Dehydrogenase
Bisbenzimidazole
DNA
Phagocytes
Plastics

ASJC Scopus subject areas

  • Hematology

Cite this

Measurement of phagocytosis utilizing [14C]cyanate-labelled human red cells and monocytes. / Bussolino, F.; Turrini, F.; Arese, P.

In: British Journal of Haematology, Vol. 66, No. 2, 1987, p. 271-274.

Research output: Contribution to journalArticle

@article{39d59a4285834ad483c140432bea54fa,
title = "Measurement of phagocytosis utilizing [14C]cyanate-labelled human red cells and monocytes",
abstract = "Human red cells were labelled with [14C]cyanate, non-oxidant, permeant reagent that binds irreversibly to amino groups in proteins. Cyanate did not modify GSH levels, nor glucose-6-phosphate dehydrogenase (G6PD) activity when added at the labelling concentration i.e. c. 0.5 mM. Phagocytes were human monocytes, isolated and plated on 16 mm diameter plastic wells. Each well was plated with 40-70x103 cells. Monocytes were quantified by DNA assay with the DNA intercalating fluorescent compound Hoechst 33258. The basal phagocytic rate of normal red cells by monocytes was 0.34±0.21 red cell per monocyte. Treatment of normal red cells with 20 μM diamide, or 50-100 μM chromate enhanced this rate 10-15-fold. 10μM diamide or chromate were sufficient to stimulate phagocytosis of G6PD-deficient (Mediterranean variant) red cells.",
author = "F. Bussolino and F. Turrini and P. Arese",
year = "1987",
language = "English",
volume = "66",
pages = "271--274",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "John Wiley & Sons, Ltd (10.1111)",
number = "2",

}

TY - JOUR

T1 - Measurement of phagocytosis utilizing [14C]cyanate-labelled human red cells and monocytes

AU - Bussolino, F.

AU - Turrini, F.

AU - Arese, P.

PY - 1987

Y1 - 1987

N2 - Human red cells were labelled with [14C]cyanate, non-oxidant, permeant reagent that binds irreversibly to amino groups in proteins. Cyanate did not modify GSH levels, nor glucose-6-phosphate dehydrogenase (G6PD) activity when added at the labelling concentration i.e. c. 0.5 mM. Phagocytes were human monocytes, isolated and plated on 16 mm diameter plastic wells. Each well was plated with 40-70x103 cells. Monocytes were quantified by DNA assay with the DNA intercalating fluorescent compound Hoechst 33258. The basal phagocytic rate of normal red cells by monocytes was 0.34±0.21 red cell per monocyte. Treatment of normal red cells with 20 μM diamide, or 50-100 μM chromate enhanced this rate 10-15-fold. 10μM diamide or chromate were sufficient to stimulate phagocytosis of G6PD-deficient (Mediterranean variant) red cells.

AB - Human red cells were labelled with [14C]cyanate, non-oxidant, permeant reagent that binds irreversibly to amino groups in proteins. Cyanate did not modify GSH levels, nor glucose-6-phosphate dehydrogenase (G6PD) activity when added at the labelling concentration i.e. c. 0.5 mM. Phagocytes were human monocytes, isolated and plated on 16 mm diameter plastic wells. Each well was plated with 40-70x103 cells. Monocytes were quantified by DNA assay with the DNA intercalating fluorescent compound Hoechst 33258. The basal phagocytic rate of normal red cells by monocytes was 0.34±0.21 red cell per monocyte. Treatment of normal red cells with 20 μM diamide, or 50-100 μM chromate enhanced this rate 10-15-fold. 10μM diamide or chromate were sufficient to stimulate phagocytosis of G6PD-deficient (Mediterranean variant) red cells.

UR - http://www.scopus.com/inward/record.url?scp=0023269282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023269282&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 271

EP - 274

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 2

ER -