Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts

U. Bottigli, P. Camici, C. R. Bellina, P. A. Salvadori, R. A. Mazzarisi, R. Barsacchi, M. Quaglia, F. Ursini

Research output: Contribution to journalArticle

Abstract

This paper describes the use of a single-photon counting apparatus (SPCA) for the continuous monitoring of the ultraweak chemiluminescence (UWC) emitted by isolated and perfused rat hearts and deriving from phospholipid peroxydation. Specifically the SPCA was used to assess the peroxidative stress induced in the heart by the addition of an hydroperoxide to the perfusing medium. To this purpose, different hydroperoxides were used and each one produced a tipical and highly reliable temporal evolution of UWC. It was also found that the oxygen availability strongly influences the intensity and the kinetic of the UWC emission. UWC was proved to be a very interesting and promising tool for monitoring the peroxidative damage of biological membranes, both because it makes a continuous assessment possible and because the sensitivity of this method is approximately ten times greater than usual analytical chemistry methods.

Original languageEnglish
Pages (from-to)936-944
Number of pages9
JournalIl Nuovo Cimento D
Volume3
Issue number5
DOIs
Publication statusPublished - May 1984

Fingerprint

Chemiluminescence
chemiluminescence
rats
Rats
Hydrogen Peroxide
counting
Photons
analytical chemistry
Biological membranes
Monitoring
Phospholipids
photons
availability
Availability
Oxygen
membranes
damage
Kinetics
sensitivity
kinetics

Keywords

  • Biophysical instrumentation and techniques

ASJC Scopus subject areas

  • Ocean Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering
  • Control and Systems Engineering
  • Engineering (miscellaneous)
  • Nuclear Energy and Engineering
  • Computer Science Applications
  • Chemical Engineering(all)

Cite this

Bottigli, U., Camici, P., Bellina, C. R., Salvadori, P. A., Mazzarisi, R. A., Barsacchi, R., ... Ursini, F. (1984). Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts. Il Nuovo Cimento D, 3(5), 936-944. https://doi.org/10.1007/BF02457093

Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts. / Bottigli, U.; Camici, P.; Bellina, C. R.; Salvadori, P. A.; Mazzarisi, R. A.; Barsacchi, R.; Quaglia, M.; Ursini, F.

In: Il Nuovo Cimento D, Vol. 3, No. 5, 05.1984, p. 936-944.

Research output: Contribution to journalArticle

Bottigli, U, Camici, P, Bellina, CR, Salvadori, PA, Mazzarisi, RA, Barsacchi, R, Quaglia, M & Ursini, F 1984, 'Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts', Il Nuovo Cimento D, vol. 3, no. 5, pp. 936-944. https://doi.org/10.1007/BF02457093
Bottigli, U. ; Camici, P. ; Bellina, C. R. ; Salvadori, P. A. ; Mazzarisi, R. A. ; Barsacchi, R. ; Quaglia, M. ; Ursini, F. / Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts. In: Il Nuovo Cimento D. 1984 ; Vol. 3, No. 5. pp. 936-944.
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