Abstract
This paper describes the use of a single-photon counting apparatus (SPCA) for the continuous monitoring of the ultraweak chemiluminescence (UWC) emitted by isolated and perfused rat hearts and deriving from phospholipid peroxydation. Specifically the SPCA was used to assess the peroxidative stress induced in the heart by the addition of an hydroperoxide to the perfusing medium. To this purpose, different hydroperoxides were used and each one produced a tipical and highly reliable temporal evolution of UWC. It was also found that the oxygen availability strongly influences the intensity and the kinetic of the UWC emission. UWC was proved to be a very interesting and promising tool for monitoring the peroxidative damage of biological membranes, both because it makes a continuous assessment possible and because the sensitivity of this method is approximately ten times greater than usual analytical chemistry methods.
| Original language | English |
|---|---|
| Pages (from-to) | 936-944 |
| Number of pages | 9 |
| Journal | Il Nuovo Cimento D |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1984 |
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Keywords
- Biophysical instrumentation and techniques
ASJC Scopus subject areas
- Ocean Engineering
- Mechanical Engineering
- Electrical and Electronic Engineering
- Control and Systems Engineering
- Engineering (miscellaneous)
- Nuclear Energy and Engineering
- Computer Science Applications
- Chemical Engineering(all)
Cite this
Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts. / Bottigli, U.; Camici, P.; Bellina, C. R.; Salvadori, P. A.; Mazzarisi, R. A.; Barsacchi, R.; Quaglia, M.; Ursini, F.
In: Il Nuovo Cimento D, Vol. 3, No. 5, 05.1984, p. 936-944.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of ultraweak chemiluminescence emitted from isolated and perfused rat hearts
AU - Bottigli, U.
AU - Camici, P.
AU - Bellina, C. R.
AU - Salvadori, P. A.
AU - Mazzarisi, R. A.
AU - Barsacchi, R.
AU - Quaglia, M.
AU - Ursini, F.
PY - 1984/5
Y1 - 1984/5
N2 - This paper describes the use of a single-photon counting apparatus (SPCA) for the continuous monitoring of the ultraweak chemiluminescence (UWC) emitted by isolated and perfused rat hearts and deriving from phospholipid peroxydation. Specifically the SPCA was used to assess the peroxidative stress induced in the heart by the addition of an hydroperoxide to the perfusing medium. To this purpose, different hydroperoxides were used and each one produced a tipical and highly reliable temporal evolution of UWC. It was also found that the oxygen availability strongly influences the intensity and the kinetic of the UWC emission. UWC was proved to be a very interesting and promising tool for monitoring the peroxidative damage of biological membranes, both because it makes a continuous assessment possible and because the sensitivity of this method is approximately ten times greater than usual analytical chemistry methods.
AB - This paper describes the use of a single-photon counting apparatus (SPCA) for the continuous monitoring of the ultraweak chemiluminescence (UWC) emitted by isolated and perfused rat hearts and deriving from phospholipid peroxydation. Specifically the SPCA was used to assess the peroxidative stress induced in the heart by the addition of an hydroperoxide to the perfusing medium. To this purpose, different hydroperoxides were used and each one produced a tipical and highly reliable temporal evolution of UWC. It was also found that the oxygen availability strongly influences the intensity and the kinetic of the UWC emission. UWC was proved to be a very interesting and promising tool for monitoring the peroxidative damage of biological membranes, both because it makes a continuous assessment possible and because the sensitivity of this method is approximately ten times greater than usual analytical chemistry methods.
KW - Biophysical instrumentation and techniques
UR - http://www.scopus.com/inward/record.url?scp=51649159030&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51649159030&partnerID=8YFLogxK
U2 - 10.1007/BF02457093
DO - 10.1007/BF02457093
M3 - Article
AN - SCOPUS:51649159030
VL - 3
SP - 936
EP - 944
JO - Nuovo Cimento della Societa Italiana di Fisica D - Condensed Matter, Atomic, Molecular and Chemical Physics, Biophysics
JF - Nuovo Cimento della Societa Italiana di Fisica D - Condensed Matter, Atomic, Molecular and Chemical Physics, Biophysics
SN - 0392-6737
IS - 5
ER -