Measurement of urinary fibrinogen-related material: methodologic aspects

Maria Benedetta Donati, Ayesha Molla, Paul Michielsen, Joseph Vermylen

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Incubation of purified plasminogen-free and plasminogen-containing fibrinogen or fibrin with normal urine concentrates revealed that urinary fibrinogenolysis depends largely on plasminogen activation; that the Staphylococcal Clumping Test (SCT) detects fibrinogen in urine concentrates and partially, but not extensively, degraded fibrinogen or fibrin derivatives; that the latter are equally reactive in the Tanned Red Cell Hemagglutination Inhibition Immunoassay (TRCHII). When urine concentrates from glomerulonephritis patients or at different intervals following renal homotransplantation were studied, an excellent correlation was found between results with the TRCHII and radial immunodiffusion; mainly urine concentrates obtained the first two months after renal homotransplantation reacted well in the SCT, suggesting nonextensive fibrin(ogen) degradation in this situation. Clottable protein was found at least once in nearly all transplanted patients; the presence of clottable protein was significantly associated with a strong reactivity in the SCT. The presence of undegraded fibrinogen in some urine concentrates was further confirmed by agarose gel chromatography. Low urinary fibrinolytic activity seems a prerequisite for the presence of clottable protein. Serum fibrin(ogen)-related antigen exceeded 4 μg per milliliter more frequently when urinary fibrin(ogen)-related antigen excretion was above 1 mg. per 24 hours. In conclusion, fibrinogen-related material in urine does not reflect only intrarenal fibrin deposition in every case. Ancillary measurements allow conclusions to be drawn on the mechanism of formation of this material in many, but not all, samples.

Original languageEnglish
Pages (from-to)921-936
Number of pages16
JournalThe Journal of Laboratory and Clinical Medicine
Issue number6
Publication statusPublished - 1974

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine


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