Measurement of warfarin in plasma by high performance liquid chromatography (HPLC) and its correlation with the international normalized ratio

Rossana Lombardi; Veena Chantarangkul; Marco Cattaneo; Armando Tripodi

doi:10.1016/j.thromres.2003.09.006

Measurement of warfarin in plasma by high performance liquid chromatography (HPLC) and its correlation with the international normalized ratio

Rossana Lombardi, Veena Chantarangkul, Marco Cattaneo, Armando Tripodi

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p

Original language	English
Pages (from-to)	281-284
Number of pages	4
Journal	Thrombosis Research
Volume	111
Issue number	4-5
DOIs	https://doi.org/10.1016/j.thromres.2003.09.006
Publication status	Published - 2003

Keywords

Anticoagulation
INR
Thrombosis
Warfarin

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine
Hematology

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10.1016/j.thromres.2003.09.006

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abstract = "The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p",

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AU - Lombardi, Rossana

AU - Chantarangkul, Veena

AU - Cattaneo, Marco

AU - Tripodi, Armando

PY - 2003

Y1 - 2003

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AB - The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p

KW - Anticoagulation

KW - INR

KW - Thrombosis

KW - Warfarin

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Measurement of warfarin in plasma by high performance liquid chromatography (HPLC) and its correlation with the international normalized ratio

Abstract

Keywords

ASJC Scopus subject areas

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