Measurement of warfarin in plasma by high performance liquid chromatography (HPLC) and its correlation with the international normalized ratio

Rossana Lombardi, Veena Chantarangkul, Marco Cattaneo, Armando Tripodi

Research output: Contribution to journalArticlepeer-review

Abstract

The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalThrombosis Research
Volume111
Issue number4-5
DOIs
Publication statusPublished - 2003

Keywords

  • Anticoagulation
  • INR
  • Thrombosis
  • Warfarin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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