Measuring ECS interaction with biomembranes

Clotilde B. Angelucci, Annalaura Sabatucci, Enrico Dainese

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Understanding the correct interaction among the different components of the endocannabinoid system (ECS) is fundamental for a proper assessment of the function of endocannabinoids (eCBs) as signaling molecules. The knowledge of how membrane environment is able to modulate intracellular trafficking of eCBs and their interacting proteins holds a huge potential in unraveling new mechanisms of ECS modulation. Here, fluorescence resonance energy transfer (FRET) technique is applied to measure the binding affinity of ECS proteins to model membranes (i.e., large unilamellar vesicles, LUVs). In particular, we describe in details the paradigmatic example of the interaction of recombinant rat FAAH-ΔTM with LUVs constituted by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages267-276
Number of pages10
Volume1412
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1412
ISSN (Print)10643745

Keywords

  • Binding isotherms
  • Biological membranes
  • FRET
  • Large unilamellar vesicles
  • Membrane affinity
  • Membrane proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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