TY - CHAP
T1 - Measuring ECS interaction with biomembranes
AU - Angelucci, Clotilde B.
AU - Sabatucci, Annalaura
AU - Dainese, Enrico
PY - 2016
Y1 - 2016
N2 - Understanding the correct interaction among the different components of the endocannabinoid system (ECS) is fundamental for a proper assessment of the function of endocannabinoids (eCBs) as signaling molecules. The knowledge of how membrane environment is able to modulate intracellular trafficking of eCBs and their interacting proteins holds a huge potential in unraveling new mechanisms of ECS modulation. Here, fluorescence resonance energy transfer (FRET) technique is applied to measure the binding affinity of ECS proteins to model membranes (i.e., large unilamellar vesicles, LUVs). In particular, we describe in details the paradigmatic example of the interaction of recombinant rat FAAH-ΔTM with LUVs constituted by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).
AB - Understanding the correct interaction among the different components of the endocannabinoid system (ECS) is fundamental for a proper assessment of the function of endocannabinoids (eCBs) as signaling molecules. The knowledge of how membrane environment is able to modulate intracellular trafficking of eCBs and their interacting proteins holds a huge potential in unraveling new mechanisms of ECS modulation. Here, fluorescence resonance energy transfer (FRET) technique is applied to measure the binding affinity of ECS proteins to model membranes (i.e., large unilamellar vesicles, LUVs). In particular, we describe in details the paradigmatic example of the interaction of recombinant rat FAAH-ΔTM with LUVs constituted by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).
KW - Binding isotherms
KW - Biological membranes
KW - FRET
KW - Large unilamellar vesicles
KW - Membrane affinity
KW - Membrane proteins
UR - http://www.scopus.com/inward/record.url?scp=84990238235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990238235&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3539-0_27
DO - 10.1007/978-1-4939-3539-0_27
M3 - Chapter
AN - SCOPUS:84990238235
VL - 1412
T3 - Methods in Molecular Biology
SP - 267
EP - 276
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -