Measuring in vivo protein half-life

Karolina Bojkowska, Francesca Santoni De Sio, Isabelle Barde, Sandra Offner, Sonia Verp, Christian Heinis, Kai Johnsson, Didier Trono

Research output: Contribution to journalArticlepeer-review

Abstract

Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and can be used for the specific in vivo labeling of SNAP-tag fusion proteins. We then apply near-infrared probes to perform noninvasive imaging of in vivo-labeled tumors. Finally, we use SNAP-mediated chemical pulse-chase labeling to perform measurement of the in vivo half-life of different extra- and intracellular proteins. These results open broad perspectives for studying protein function in living animals.

Original languageEnglish
Pages (from-to)805-815
Number of pages11
JournalChemistry and Biology
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 24 2011

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Molecular Biology
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Medicine(all)

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