Measuring microRNA expression levels in oncology: From samples to data analysis

Loris De Cecco, Matteo Dugo, Silvana Canevari, Maria Grazia Daidone, Maurizio Callari

Research output: Contribution to journalArticlepeer-review

Abstract

The relevance of microRNAs (miRNAs) in mammalian species was highlighted in the past decade. Inherent to the rapid advancements in the miRNA field was the growing need for applicable and validated experimental tools that enabled rapid progress from discovery to development of diagnostic and therapeutic applications. In the last few years, different technologies have been developed to measure miRNA expression, and one important challenge is making sense of miRNA profiles. In fact, technical and analytical aspects have substantial impact on subsequent biological interpretations and their translational applications. In the context of the workflow of the entire process of miRNA analysis, we will herein (i) consider some of the more relevant pre-analytical aspects such as study design, sample processing, and miRNA extraction, (ii) describe the available analytical tools for miRNA profiling (reverse transcription quantitative PCR, microarray hybridization, next-generation sequencing) and discuss their strengths and weaknesses, (iii) highlight the available postanalytical strategies for quality control, normalization, and statistical and bioinformatic analyses of miRNA profiling data, and (iv) summarize cross-platform comparison studies.

Original languageEnglish
Pages (from-to)273-287
Number of pages15
JournalCritical Reviews in Oncogenesis
Volume18
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • Computational aspects
  • Cross-platform comparison
  • Microarray
  • microRNA
  • Quality control
  • Reverse transcription quantitative PCR
  • RNA sequencing

ASJC Scopus subject areas

  • Cancer Research

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