Measuring the confluence of iPSCs using an automated imaging system

Valentina Magliocca, Maria Vinci, Tiziana Persichini, Franco Locatelli, Marco Tartaglia, Claudia Compagnucci

Research output: Contribution to journalArticlepeer-review

Abstract

This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single cell suspension to avoid any growth perturbation. A high-content image analysis system was used to assess iPCS confluence on 4 different ECMs over time in an automated manner. Different analysis settings were used to assess cell confluence of adherent iPSCs and only a slight difference (at 24 and 48 hours with laminin) has been observed whether a 60, 80 or 100% mask was applied. We also show that laminin lead to the best confluence compared to Matrigel, vitronectin and fibronectin.

Original languageEnglish
Article numbere61225
Pages (from-to)1-10
Number of pages10
JournalJournal of Visualized Experiments
Volume2020
Issue number160
DOIs
Publication statusPublished - Jun 2020

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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