Abstract
The adipose tissue is a source of inflammatory proteins, such as TNF, IL-6, and CXCL8. Most of their production occurs in macrophages that act as scavengers of dying adipocytes. The application of an orbital mechanical force for 6-10 min at 97 g to the adipose tissue, lipoaspirated and treated according to Coleman procedures, abolishes the expression of TNF-α and stimulates the expression of the anti-inflammatory protein TNF-stimulated gene-6 (TSG-6). This protein had protective and anti-inflammatory effects when applied to animal models of rheumatic diseases. We examined biopsy, lipoaspirate, and mechanically activated fat and observed that in addition to the increased TSG-6, Sox2, Nanog, and Oct4 were also strongly augmented by mechanical activation, suggesting an effect on stromal cell stemness. Human adipose tissue-derived mesenchymal stem cells (hADSCs), produced from activated fat, grow and differentiate normally with proper cell surface markers and chromosomal integrity, but their anti-inflammatory action is far superior compared to those mesenchymal stem cells (MSCs) obtained from lipoaspirate. The expression and release of inflammatory cytokines from THP-1 cells was totally abolished in mechanically activated adipose tissue-derived hADSCs. In conclusion, we report that the orbital shaking of adipose tissue enhances its anti-inflammatory properties, and derived MSCs maintain such enhanced activity.
| Original language | English |
|---|---|
| Journal | International Journal of Molecular Sciences |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 16 2018 |
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Keywords
- Adipogenesis
- Adipose Tissue/cytology
- Anti-Inflammatory Agents/metabolism
- Cell Separation
- Cells, Cultured
- Cytokines/genetics
- Gene Expression Regulation
- Humans
- Lipectomy
- Mesenchymal Stem Cells/cytology
- Osteogenesis
- Pluripotent Stem Cells/metabolism
- Stress, Mechanical
Cite this
Mechanical Activation of Adipose Tissue and Derived Mesenchymal Stem Cells : Novel Anti-Inflammatory Properties. / Carelli, Stephana; Colli, Mattia; Vinci, Valeriano; Caviggioli, Fabio; Klinger, Marco; Gorio, Alfredo.
In: International Journal of Molecular Sciences, Vol. 19, No. 1, 16.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanical Activation of Adipose Tissue and Derived Mesenchymal Stem Cells
T2 - Novel Anti-Inflammatory Properties
AU - Carelli, Stephana
AU - Colli, Mattia
AU - Vinci, Valeriano
AU - Caviggioli, Fabio
AU - Klinger, Marco
AU - Gorio, Alfredo
PY - 2018/1/16
Y1 - 2018/1/16
N2 - The adipose tissue is a source of inflammatory proteins, such as TNF, IL-6, and CXCL8. Most of their production occurs in macrophages that act as scavengers of dying adipocytes. The application of an orbital mechanical force for 6-10 min at 97 g to the adipose tissue, lipoaspirated and treated according to Coleman procedures, abolishes the expression of TNF-α and stimulates the expression of the anti-inflammatory protein TNF-stimulated gene-6 (TSG-6). This protein had protective and anti-inflammatory effects when applied to animal models of rheumatic diseases. We examined biopsy, lipoaspirate, and mechanically activated fat and observed that in addition to the increased TSG-6, Sox2, Nanog, and Oct4 were also strongly augmented by mechanical activation, suggesting an effect on stromal cell stemness. Human adipose tissue-derived mesenchymal stem cells (hADSCs), produced from activated fat, grow and differentiate normally with proper cell surface markers and chromosomal integrity, but their anti-inflammatory action is far superior compared to those mesenchymal stem cells (MSCs) obtained from lipoaspirate. The expression and release of inflammatory cytokines from THP-1 cells was totally abolished in mechanically activated adipose tissue-derived hADSCs. In conclusion, we report that the orbital shaking of adipose tissue enhances its anti-inflammatory properties, and derived MSCs maintain such enhanced activity.
AB - The adipose tissue is a source of inflammatory proteins, such as TNF, IL-6, and CXCL8. Most of their production occurs in macrophages that act as scavengers of dying adipocytes. The application of an orbital mechanical force for 6-10 min at 97 g to the adipose tissue, lipoaspirated and treated according to Coleman procedures, abolishes the expression of TNF-α and stimulates the expression of the anti-inflammatory protein TNF-stimulated gene-6 (TSG-6). This protein had protective and anti-inflammatory effects when applied to animal models of rheumatic diseases. We examined biopsy, lipoaspirate, and mechanically activated fat and observed that in addition to the increased TSG-6, Sox2, Nanog, and Oct4 were also strongly augmented by mechanical activation, suggesting an effect on stromal cell stemness. Human adipose tissue-derived mesenchymal stem cells (hADSCs), produced from activated fat, grow and differentiate normally with proper cell surface markers and chromosomal integrity, but their anti-inflammatory action is far superior compared to those mesenchymal stem cells (MSCs) obtained from lipoaspirate. The expression and release of inflammatory cytokines from THP-1 cells was totally abolished in mechanically activated adipose tissue-derived hADSCs. In conclusion, we report that the orbital shaking of adipose tissue enhances its anti-inflammatory properties, and derived MSCs maintain such enhanced activity.
KW - Adipogenesis
KW - Adipose Tissue/cytology
KW - Anti-Inflammatory Agents/metabolism
KW - Cell Separation
KW - Cells, Cultured
KW - Cytokines/genetics
KW - Gene Expression Regulation
KW - Humans
KW - Lipectomy
KW - Mesenchymal Stem Cells/cytology
KW - Osteogenesis
KW - Pluripotent Stem Cells/metabolism
KW - Stress, Mechanical
U2 - 10.3390/ijms19010267
DO - 10.3390/ijms19010267
M3 - Article
C2 - 29337886
VL - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 1
ER -