Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines

Giorgio Brandi, Giuditta F. Schiavano, Nadia Zaffaroni, Cinzia De Marco, Mirko Paiardini, Barbara Cervasi, Mauro Magnani

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Cruciferous vegetables are an important source of compounds that may be useful for chemoprevention. In this study, we evaluated the antiproliferative activity of juice obtained from leaves of several varieties of Brassica oleracea on both estrogen receptor (ER)-positive (ER+; MCF-7 and BT474) and ER-negative (ER-; MDA-MB-231 and BT20) human breast cancer cell lines. The effect of juice on cell proliferation was evaluated on DNA synthesis and on cell cycle-related proteins. Juice markedly reduced DNA synthesis, evaluated by [ 3H]thymidine incorporation, starting from low concentrations (final concentration 5-15 mL/L), and this activity was independent of ER. All cauliflower varieties tested suppressed cell proliferation in a dose-dependent manner. Cell growth inhibition was accompanied by significant cell death at the higher juice concentrations, although no evidence of apoptosis was found. Interestingly, the juice displayed a preferential activity against breast cancer cells compared with other mammalian cell lines investigated (ECV304, VERO, Hep2, 3T3, and MCF-10A) (P <0.01). At the molecular level, the inhibition of proliferation was associated with significantly reduced CDK6 expression and an increased level of p27 in ER+ cells but not in ER- cells, whereas a common feature in all cell lines was significantly decreased retinoblastoma protein phosphorylation. These results suggest that the edible part of Brassica oleracea contains substances that can markedly inhibit the growth of both ER+ and ER- human breast cancer cells, although through different mechanisms. These results suggest that the widely available cruciferous vegetables are potential chemopreventive agents.

Original languageEnglish
Pages (from-to)1503-1509
Number of pages7
JournalJournal of Nutrition
Volume135
Issue number6
Publication statusPublished - Jun 2005

Fingerprint

Brassica
Brassica oleracea
Estrogen Receptors
breast neoplasms
juices
mechanism of action
cell lines
Breast Neoplasms
Cell Line
Vegetables
cell proliferation
vegetables
Cell Proliferation
neoplasm cells
estrogen receptors
Retinoblastoma Protein
Cell Cycle Proteins
chemoprevention
synthesis
protein phosphorylation

Keywords

  • Brassica juice
  • Breast cancer
  • CDK6
  • Chemoprevention
  • p27

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Brandi, G., Schiavano, G. F., Zaffaroni, N., De Marco, C., Paiardini, M., Cervasi, B., & Magnani, M. (2005). Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines. Journal of Nutrition, 135(6), 1503-1509.

Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines. / Brandi, Giorgio; Schiavano, Giuditta F.; Zaffaroni, Nadia; De Marco, Cinzia; Paiardini, Mirko; Cervasi, Barbara; Magnani, Mauro.

In: Journal of Nutrition, Vol. 135, No. 6, 06.2005, p. 1503-1509.

Research output: Contribution to journalArticle

Brandi, G, Schiavano, GF, Zaffaroni, N, De Marco, C, Paiardini, M, Cervasi, B & Magnani, M 2005, 'Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines', Journal of Nutrition, vol. 135, no. 6, pp. 1503-1509.
Brandi, Giorgio ; Schiavano, Giuditta F. ; Zaffaroni, Nadia ; De Marco, Cinzia ; Paiardini, Mirko ; Cervasi, Barbara ; Magnani, Mauro. / Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines. In: Journal of Nutrition. 2005 ; Vol. 135, No. 6. pp. 1503-1509.
@article{8afd4377c874445b832c815578119350,
title = "Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines",
abstract = "Cruciferous vegetables are an important source of compounds that may be useful for chemoprevention. In this study, we evaluated the antiproliferative activity of juice obtained from leaves of several varieties of Brassica oleracea on both estrogen receptor (ER)-positive (ER+; MCF-7 and BT474) and ER-negative (ER-; MDA-MB-231 and BT20) human breast cancer cell lines. The effect of juice on cell proliferation was evaluated on DNA synthesis and on cell cycle-related proteins. Juice markedly reduced DNA synthesis, evaluated by [ 3H]thymidine incorporation, starting from low concentrations (final concentration 5-15 mL/L), and this activity was independent of ER. All cauliflower varieties tested suppressed cell proliferation in a dose-dependent manner. Cell growth inhibition was accompanied by significant cell death at the higher juice concentrations, although no evidence of apoptosis was found. Interestingly, the juice displayed a preferential activity against breast cancer cells compared with other mammalian cell lines investigated (ECV304, VERO, Hep2, 3T3, and MCF-10A) (P <0.01). At the molecular level, the inhibition of proliferation was associated with significantly reduced CDK6 expression and an increased level of p27 in ER+ cells but not in ER- cells, whereas a common feature in all cell lines was significantly decreased retinoblastoma protein phosphorylation. These results suggest that the edible part of Brassica oleracea contains substances that can markedly inhibit the growth of both ER+ and ER- human breast cancer cells, although through different mechanisms. These results suggest that the widely available cruciferous vegetables are potential chemopreventive agents.",
keywords = "Brassica juice, Breast cancer, CDK6, Chemoprevention, p27",
author = "Giorgio Brandi and Schiavano, {Giuditta F.} and Nadia Zaffaroni and {De Marco}, Cinzia and Mirko Paiardini and Barbara Cervasi and Mauro Magnani",
year = "2005",
month = "6",
language = "English",
volume = "135",
pages = "1503--1509",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "6",

}

TY - JOUR

T1 - Mechanisms of action and antiproliferative properties of Brassica oleracea juice in human breast cancer cell lines

AU - Brandi, Giorgio

AU - Schiavano, Giuditta F.

AU - Zaffaroni, Nadia

AU - De Marco, Cinzia

AU - Paiardini, Mirko

AU - Cervasi, Barbara

AU - Magnani, Mauro

PY - 2005/6

Y1 - 2005/6

N2 - Cruciferous vegetables are an important source of compounds that may be useful for chemoprevention. In this study, we evaluated the antiproliferative activity of juice obtained from leaves of several varieties of Brassica oleracea on both estrogen receptor (ER)-positive (ER+; MCF-7 and BT474) and ER-negative (ER-; MDA-MB-231 and BT20) human breast cancer cell lines. The effect of juice on cell proliferation was evaluated on DNA synthesis and on cell cycle-related proteins. Juice markedly reduced DNA synthesis, evaluated by [ 3H]thymidine incorporation, starting from low concentrations (final concentration 5-15 mL/L), and this activity was independent of ER. All cauliflower varieties tested suppressed cell proliferation in a dose-dependent manner. Cell growth inhibition was accompanied by significant cell death at the higher juice concentrations, although no evidence of apoptosis was found. Interestingly, the juice displayed a preferential activity against breast cancer cells compared with other mammalian cell lines investigated (ECV304, VERO, Hep2, 3T3, and MCF-10A) (P <0.01). At the molecular level, the inhibition of proliferation was associated with significantly reduced CDK6 expression and an increased level of p27 in ER+ cells but not in ER- cells, whereas a common feature in all cell lines was significantly decreased retinoblastoma protein phosphorylation. These results suggest that the edible part of Brassica oleracea contains substances that can markedly inhibit the growth of both ER+ and ER- human breast cancer cells, although through different mechanisms. These results suggest that the widely available cruciferous vegetables are potential chemopreventive agents.

AB - Cruciferous vegetables are an important source of compounds that may be useful for chemoprevention. In this study, we evaluated the antiproliferative activity of juice obtained from leaves of several varieties of Brassica oleracea on both estrogen receptor (ER)-positive (ER+; MCF-7 and BT474) and ER-negative (ER-; MDA-MB-231 and BT20) human breast cancer cell lines. The effect of juice on cell proliferation was evaluated on DNA synthesis and on cell cycle-related proteins. Juice markedly reduced DNA synthesis, evaluated by [ 3H]thymidine incorporation, starting from low concentrations (final concentration 5-15 mL/L), and this activity was independent of ER. All cauliflower varieties tested suppressed cell proliferation in a dose-dependent manner. Cell growth inhibition was accompanied by significant cell death at the higher juice concentrations, although no evidence of apoptosis was found. Interestingly, the juice displayed a preferential activity against breast cancer cells compared with other mammalian cell lines investigated (ECV304, VERO, Hep2, 3T3, and MCF-10A) (P <0.01). At the molecular level, the inhibition of proliferation was associated with significantly reduced CDK6 expression and an increased level of p27 in ER+ cells but not in ER- cells, whereas a common feature in all cell lines was significantly decreased retinoblastoma protein phosphorylation. These results suggest that the edible part of Brassica oleracea contains substances that can markedly inhibit the growth of both ER+ and ER- human breast cancer cells, although through different mechanisms. These results suggest that the widely available cruciferous vegetables are potential chemopreventive agents.

KW - Brassica juice

KW - Breast cancer

KW - CDK6

KW - Chemoprevention

KW - p27

UR - http://www.scopus.com/inward/record.url?scp=20444372721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444372721&partnerID=8YFLogxK

M3 - Article

VL - 135

SP - 1503

EP - 1509

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 6

ER -