Mechanisms of the interaction between two ADAMTS13 gene mutations leading to severe deficiency of enzymatic activity

Flora Peyvandi, Silvia Lavoretano, Roberta Palla, Carla Valsecchi, Giuliana Merati, Raimondo De Cristofaro, Edoardo Rossi, Pier Mannuccio Mannucci

Research output: Contribution to journalArticlepeer-review

Abstract

The inherited deficiency of the von Willebrand factor-cleaving protease ADAMTS13 is associated with rare forms of thrombotic thrombocytopenic purpura (TTP). We investigated a woman with a family history of chronic recurrent TTP and undetectable plasma levels of ADAMTS13 activity. Genetic analysis revealed two missense mutations in the heterozygous state: p.Val88Met substitution in the metalloprotease domain and p.Gly1239Val substitution in the first CUB domain of ADAMTS13. To explore the mechanism of ADAMTS13 deficiency in this patient, the wild type (WT; ADAMT13WT) and each mutant construct (ADAMTS13 Val88Met, ADAMTS13Gly1239Val) were transiently expressed in HEK 293 and COS-7 cells. To recapitulate the compound heterozygous state of the patient, both mutant ADAMTS13 proteins were also expressed together. The p.Val88Met mutation led to a defect of secretion of the protease associated with a reduction of enzymatic activity, the p.Gly1239Val mutation led to a secretion defect causing intracellular accumulation of the protease. The mechanistic effects of the mutations were further explored by means of differential immunofluorescence, that demonstrated an homogeneous distribution of ADAMTS13WT in the Cis-Golgi and endoplasmic reticulum (ER) compartments, a reduction of ADAMTS13Val88Met in both compartments, while ADAMTS13Gly1239Val failed to reach the Cis-Golgi compartment and remained in the ER.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalHuman Mutation
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 2006

Keywords

  • ADAMTS13
  • Mutations expression study
  • Thrombotic thrombocytopenic purpura (TTP)

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Mechanisms of the interaction between two ADAMTS13 gene mutations leading to severe deficiency of enzymatic activity'. Together they form a unique fingerprint.

Cite this