Mechanosensory genes Pkd1 and Pkd2 contribute to the planar polarization of brain ventricular epithelium

Shinya Ohata, Vicente Herranz-Pérez, Jin Nakatani, Alessandra Boletta, José Manuel García-Verdugo, Arturo Álvarez-Buylla

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Directional beating of ependymal (E) cells’ cilia in the walls of the ventricles in the brain is essential for properCSFflow.Ecells display two forms of planar cell polarity (PCP): rotational polarity of individual cilium and translational polarity(asymmetricpositioning of cilia in the apicalarea). Theorientation of individualEcells variesaccordingto their location in the ventricular wall (location-specificPCP).Ithasbeenhypothesizedthat hydrodynamic forcesonthe apical surface of radial glia cells (RGCs), theembryonic precursors of Ecells, could guide location-specificPCPin the ventricular epithelium. However, the detection mechanisms for these hydrodynamic forces have not been identified. Here, we show that the mechanosensory proteins polycystic kidney disease 1 (Pkd1) and Pkd2 are present in primary cilia of RGCs. Ablation of Pkd1 or Pkd2 in Nestin-Cre;Pkd1flox/flox or Nestin-Cre;Pkd2flox/flox mice, affected PCP development in RGCs and E cells. Early shear forces on the ventricular epitheliummayactivatePkd1andPkd2in primary cilia ofRGCsto properly polarizeRGCsandEcells. Consistently, Pkd1, Pkd2, or primary cilia on RGCs were required for the proper asymmetric localization of the PCP protein Vangl2 in E cells’ apical area. Analyses of single- and doubleheterozygous mutants for Pkd1 and/or Vangl2 suggest that these genes function in the same pathway to establish E cells’ PCP.Weconclude that Pkd1 and Pkd2 mechanosensory proteins contribute to the development of brain PCP and prevention of hydrocephalus.

Original languageEnglish
Pages (from-to)11153-11168
Number of pages16
JournalJournal of Neuroscience
Volume35
Issue number31
DOIs
Publication statusPublished - Aug 5 2015

Fingerprint

Polycystic Kidney Diseases
Cilia
Epithelium
Cell Polarity
Brain
Neuroglia
Genes
Nestin
Hydrodynamics
Body Patterning
Hydrocephalus
Proteins

Keywords

  • Cilia
  • Ependymal cell
  • Epithelium
  • Neural stem cell
  • Planar cell polarity
  • Polycystin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ohata, S., Herranz-Pérez, V., Nakatani, J., Boletta, A., García-Verdugo, J. M., & Álvarez-Buylla, A. (2015). Mechanosensory genes Pkd1 and Pkd2 contribute to the planar polarization of brain ventricular epithelium. Journal of Neuroscience, 35(31), 11153-11168. https://doi.org/10.1523/JNEUROSCI.0686-15.2015

Mechanosensory genes Pkd1 and Pkd2 contribute to the planar polarization of brain ventricular epithelium. / Ohata, Shinya; Herranz-Pérez, Vicente; Nakatani, Jin; Boletta, Alessandra; García-Verdugo, José Manuel; Álvarez-Buylla, Arturo.

In: Journal of Neuroscience, Vol. 35, No. 31, 05.08.2015, p. 11153-11168.

Research output: Contribution to journalArticle

Ohata, S, Herranz-Pérez, V, Nakatani, J, Boletta, A, García-Verdugo, JM & Álvarez-Buylla, A 2015, 'Mechanosensory genes Pkd1 and Pkd2 contribute to the planar polarization of brain ventricular epithelium', Journal of Neuroscience, vol. 35, no. 31, pp. 11153-11168. https://doi.org/10.1523/JNEUROSCI.0686-15.2015
Ohata, Shinya ; Herranz-Pérez, Vicente ; Nakatani, Jin ; Boletta, Alessandra ; García-Verdugo, José Manuel ; Álvarez-Buylla, Arturo. / Mechanosensory genes Pkd1 and Pkd2 contribute to the planar polarization of brain ventricular epithelium. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 31. pp. 11153-11168.
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