MeCP2 related studies benefit from the use of CD1 as genetic background

Clementina Cobolli Gigli, Linda Scaramuzza, Anna Gandaglia, Elisa Bellini, Marina Gabaglio, Daniela Parolaro, Charlotte Kilstrup-Nielsen, Nicoletta Landsberger, Francesco Bedogni

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

MECP2 mutations cause a number of neurological disorders of which Rett syndrome (RTT) represents the most thoroughly analysed condition. Many Mecp2 mouse models have been generated through the years; their validity is demonstrated by the presence of a broad spectrum of phenotypes largely mimicking those manifested by RTT patients. These mouse models, between which the C57BL/6 Mecp2tm1.1Bird strain probably represents the most used, enabled to disclose much of the roles of Mecp2. However, small litters with little viability and poor maternal care hamper the maintenance of the colony, thus limiting research on such animals. For this reason, past studies often used Mecp2 mouse models on mixed genetic backgrounds, thus opening questions on whether modifier genes could be responsible for at least part of the described effects. To verify this possibility, and facilitate the maintenance of the Mecp2 colony, we transferred the Mecp2tm1.1Bird allele on the stronger CD1 background. The CD1 strain is easier to maintain and largely recapitulates the phenotypes already described in Mecp2-null mice. We believe that this mouse model will foster the research on RTT.

Original languageEnglish
Article numbere0153473
JournalPLoS One
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 1 2016

Fingerprint

genetic background
Rett Syndrome
animal models
phenotype
modifiers (genes)
nervous system diseases
Maintenance
Modifier Genes
Phenotype
Animals
Nervous System Diseases
Research
viability
alleles
mutation
Alleles
Mothers
Genetic Background
mice
Mutation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gigli, C. C., Scaramuzza, L., Gandaglia, A., Bellini, E., Gabaglio, M., Parolaro, D., ... Bedogni, F. (2016). MeCP2 related studies benefit from the use of CD1 as genetic background. PLoS One, 11(4), [e0153473]. https://doi.org/10.1371/journal.pone.0153473

MeCP2 related studies benefit from the use of CD1 as genetic background. / Gigli, Clementina Cobolli; Scaramuzza, Linda; Gandaglia, Anna; Bellini, Elisa; Gabaglio, Marina; Parolaro, Daniela; Kilstrup-Nielsen, Charlotte; Landsberger, Nicoletta; Bedogni, Francesco.

In: PLoS One, Vol. 11, No. 4, e0153473, 01.04.2016.

Research output: Contribution to journalArticle

Gigli, CC, Scaramuzza, L, Gandaglia, A, Bellini, E, Gabaglio, M, Parolaro, D, Kilstrup-Nielsen, C, Landsberger, N & Bedogni, F 2016, 'MeCP2 related studies benefit from the use of CD1 as genetic background', PLoS One, vol. 11, no. 4, e0153473. https://doi.org/10.1371/journal.pone.0153473
Gigli CC, Scaramuzza L, Gandaglia A, Bellini E, Gabaglio M, Parolaro D et al. MeCP2 related studies benefit from the use of CD1 as genetic background. PLoS One. 2016 Apr 1;11(4). e0153473. https://doi.org/10.1371/journal.pone.0153473
Gigli, Clementina Cobolli ; Scaramuzza, Linda ; Gandaglia, Anna ; Bellini, Elisa ; Gabaglio, Marina ; Parolaro, Daniela ; Kilstrup-Nielsen, Charlotte ; Landsberger, Nicoletta ; Bedogni, Francesco. / MeCP2 related studies benefit from the use of CD1 as genetic background. In: PLoS One. 2016 ; Vol. 11, No. 4.
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