Mediator release after endobronchial antigen challenge in patients with respiratory allergy

Antonio Miadonna, Alberto Tedeschi, Cesare Brasca, Giancarlo Folco, Angelo Sala, Robert C. Murphy

Research output: Contribution to journalArticle

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Abstract

The aim of the present study was to evaluate the release of some potential mediators of allergic reactions, such as histamine, peptide leukotrienes (LTs), LTB4 and prostaglandin D2 (PGD2), in bronchoalveolar lavage (BAL) fluids from 11 patients with respiratory allergy (eight with bronchial asthma and three with allergic rhinitis), who underwent specific endobronchial challenge. Histamine, peptide LT, and PGD2 levels in BAL fluids increased significantly after antigen stimulation both in patients with asthma and in patients with rhinitis. By contrast, LTB4 concentration was always below the limits of detection of the radioimmunoassay. In patients with asthma, histamine concentration increased from 5.3 ± 0.6 ng/ml in lavages obtained before provocation to 20.2 ± 5.8 ng/ml (mean ± SEM; p <0.04) 5 minutes after bronchoprovocation. Peptide LTs increased from 0.32 ± 0.08 to 0.82 ± 0.21 ng/ml (p <0.02) and PGD2 from 0.06 ± 0.01 ng/ml to 0.36 ± 0.09 ng/ml (p <0.02). Elevated histamine, peptide LT, and PGD2 concentrations were also found in the 15-minute postchallenge BAL fluids. Similar results were obtained in patients with rhinitis. Histamine concentration was 3.4 ± 0.6 ng/ml in prechallenge bronchial lavages and 11.3 ± 1.7 ng/ml in postchallenge lavages; peptide LTs increased from 0.13 ± 0.008 ng/ml to 0.73 ± 0.21 ng/ml, and PGD2 from 0.05 ± 0.01 ng/ml to 0.26 ± 0.06 ng/ml. Histamine, peptide LTs, and PGD2 were released into bronchial airways after local antigen challenge in all 11 subjects with respiratory allergy, whereas in three normal subjects (not allergic to the antigen used), there was no release of these mediators into BAL fluid above their basal concentrations. These findings, combined with known effects on human airways, suggest that histamine, peptide LTs, and PGD2 may be important in the pathogenesis of early antigen-induced asthmatic reactions.

Original languageEnglish
Pages (from-to)906-913
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume85
Issue number5
DOIs
Publication statusPublished - 1990

Fingerprint

Prostaglandin D2
Leukotrienes
Histamine
Hypersensitivity
Antigens
Bronchoalveolar Lavage Fluid
Peptides
Leukotriene B4
Asthma
Therapeutic Irrigation
Rhinitis
Bronchoalveolar Lavage
Radioimmunoassay
Limit of Detection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Mediator release after endobronchial antigen challenge in patients with respiratory allergy. / Miadonna, Antonio; Tedeschi, Alberto; Brasca, Cesare; Folco, Giancarlo; Sala, Angelo; Murphy, Robert C.

In: Journal of Allergy and Clinical Immunology, Vol. 85, No. 5, 1990, p. 906-913.

Research output: Contribution to journalArticle

Miadonna, Antonio ; Tedeschi, Alberto ; Brasca, Cesare ; Folco, Giancarlo ; Sala, Angelo ; Murphy, Robert C. / Mediator release after endobronchial antigen challenge in patients with respiratory allergy. In: Journal of Allergy and Clinical Immunology. 1990 ; Vol. 85, No. 5. pp. 906-913.
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abstract = "The aim of the present study was to evaluate the release of some potential mediators of allergic reactions, such as histamine, peptide leukotrienes (LTs), LTB4 and prostaglandin D2 (PGD2), in bronchoalveolar lavage (BAL) fluids from 11 patients with respiratory allergy (eight with bronchial asthma and three with allergic rhinitis), who underwent specific endobronchial challenge. Histamine, peptide LT, and PGD2 levels in BAL fluids increased significantly after antigen stimulation both in patients with asthma and in patients with rhinitis. By contrast, LTB4 concentration was always below the limits of detection of the radioimmunoassay. In patients with asthma, histamine concentration increased from 5.3 ± 0.6 ng/ml in lavages obtained before provocation to 20.2 ± 5.8 ng/ml (mean ± SEM; p <0.04) 5 minutes after bronchoprovocation. Peptide LTs increased from 0.32 ± 0.08 to 0.82 ± 0.21 ng/ml (p <0.02) and PGD2 from 0.06 ± 0.01 ng/ml to 0.36 ± 0.09 ng/ml (p <0.02). Elevated histamine, peptide LT, and PGD2 concentrations were also found in the 15-minute postchallenge BAL fluids. Similar results were obtained in patients with rhinitis. Histamine concentration was 3.4 ± 0.6 ng/ml in prechallenge bronchial lavages and 11.3 ± 1.7 ng/ml in postchallenge lavages; peptide LTs increased from 0.13 ± 0.008 ng/ml to 0.73 ± 0.21 ng/ml, and PGD2 from 0.05 ± 0.01 ng/ml to 0.26 ± 0.06 ng/ml. Histamine, peptide LTs, and PGD2 were released into bronchial airways after local antigen challenge in all 11 subjects with respiratory allergy, whereas in three normal subjects (not allergic to the antigen used), there was no release of these mediators into BAL fluid above their basal concentrations. These findings, combined with known effects on human airways, suggest that histamine, peptide LTs, and PGD2 may be important in the pathogenesis of early antigen-induced asthmatic reactions.",
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