Medium-term cyclosporin renal dysfunction and its reversibility in rats

Massimo Sabbatini, Luca De Nicola, Francesco Uccello, Giulio Romano, Giampaolo Papaccio, Vincenzo Esposito, Vincenzo Sepe, Giuseppe Conte, Giorgio Fuiano

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10%, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45%, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26%, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8%, P <0.05), the increase of afferent arteriole resistance (Ra; +40%, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28%, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume260
Issue number6 29-6
Publication statusPublished - 1991

Fingerprint

Cyclosporine
Nephrons
Glomerular Filtration Rate
Kidney
Punctures
Electron Microscopy
Plasma Volume
Arterioles
Weight Gain
Reference Values
Sodium
Body Weight
Pressure
Olive Oil
aliflurane

Keywords

  • Micropuncture
  • Nephrotoxicity

ASJC Scopus subject areas

  • Physiology

Cite this

Sabbatini, M., De Nicola, L., Uccello, F., Romano, G., Papaccio, G., Esposito, V., ... Fuiano, G. (1991). Medium-term cyclosporin renal dysfunction and its reversibility in rats. American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 260(6 29-6).

Medium-term cyclosporin renal dysfunction and its reversibility in rats. / Sabbatini, Massimo; De Nicola, Luca; Uccello, Francesco; Romano, Giulio; Papaccio, Giampaolo; Esposito, Vincenzo; Sepe, Vincenzo; Conte, Giuseppe; Fuiano, Giorgio.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 260, No. 6 29-6, 1991.

Research output: Contribution to journalArticle

Sabbatini, M, De Nicola, L, Uccello, F, Romano, G, Papaccio, G, Esposito, V, Sepe, V, Conte, G & Fuiano, G 1991, 'Medium-term cyclosporin renal dysfunction and its reversibility in rats', American Journal of Physiology - Renal Fluid and Electrolyte Physiology, vol. 260, no. 6 29-6.
Sabbatini M, De Nicola L, Uccello F, Romano G, Papaccio G, Esposito V et al. Medium-term cyclosporin renal dysfunction and its reversibility in rats. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1991;260(6 29-6).
Sabbatini, Massimo ; De Nicola, Luca ; Uccello, Francesco ; Romano, Giulio ; Papaccio, Giampaolo ; Esposito, Vincenzo ; Sepe, Vincenzo ; Conte, Giuseppe ; Fuiano, Giorgio. / Medium-term cyclosporin renal dysfunction and its reversibility in rats. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1991 ; Vol. 260, No. 6 29-6.
@article{d883357b792f4a81adc87d71d2067120,
title = "Medium-term cyclosporin renal dysfunction and its reversibility in rats",
abstract = "Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10{\%}, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45{\%}, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26{\%}, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8{\%}, P <0.05), the increase of afferent arteriole resistance (Ra; +40{\%}, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28{\%}, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.",
keywords = "Micropuncture, Nephrotoxicity",
author = "Massimo Sabbatini and {De Nicola}, Luca and Francesco Uccello and Giulio Romano and Giampaolo Papaccio and Vincenzo Esposito and Vincenzo Sepe and Giuseppe Conte and Giorgio Fuiano",
year = "1991",
language = "English",
volume = "260",
journal = "American Journal of Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "6 29-6",

}

TY - JOUR

T1 - Medium-term cyclosporin renal dysfunction and its reversibility in rats

AU - Sabbatini, Massimo

AU - De Nicola, Luca

AU - Uccello, Francesco

AU - Romano, Giulio

AU - Papaccio, Giampaolo

AU - Esposito, Vincenzo

AU - Sepe, Vincenzo

AU - Conte, Giuseppe

AU - Fuiano, Giorgio

PY - 1991

Y1 - 1991

N2 - Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10%, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45%, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26%, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8%, P <0.05), the increase of afferent arteriole resistance (Ra; +40%, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28%, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.

AB - Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10%, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45%, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26%, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8%, P <0.05), the increase of afferent arteriole resistance (Ra; +40%, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28%, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.

KW - Micropuncture

KW - Nephrotoxicity

UR - http://www.scopus.com/inward/record.url?scp=0025827435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025827435&partnerID=8YFLogxK

M3 - Article

VL - 260

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6119

IS - 6 29-6

ER -