Abstract
Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10%, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45%, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26%, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8%, P <0.05), the increase of afferent arteriole resistance (Ra; +40%, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28%, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Renal Fluid and Electrolyte Physiology |
| Volume | 260 |
| Issue number | 6 29-6 |
| Publication status | Published - 1991 |
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Keywords
- Micropuncture
- Nephrotoxicity
ASJC Scopus subject areas
- Physiology
Cite this
Medium-term cyclosporin renal dysfunction and its reversibility in rats. / Sabbatini, Massimo; De Nicola, Luca; Uccello, Francesco; Romano, Giulio; Papaccio, Giampaolo; Esposito, Vincenzo; Sepe, Vincenzo; Conte, Giuseppe; Fuiano, Giorgio.
In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 260, No. 6 29-6, 1991.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Medium-term cyclosporin renal dysfunction and its reversibility in rats
AU - Sabbatini, Massimo
AU - De Nicola, Luca
AU - Uccello, Francesco
AU - Romano, Giulio
AU - Papaccio, Giampaolo
AU - Esposito, Vincenzo
AU - Sepe, Vincenzo
AU - Conte, Giuseppe
AU - Fuiano, Giorgio
PY - 1991
Y1 - 1991
N2 - Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10%, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45%, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26%, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8%, P <0.05), the increase of afferent arteriole resistance (Ra; +40%, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28%, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.
AB - Renal dynamics and morphol-ogy were investigated by metabolic, renal micropuncture (RM), and electron microscopy (EM) studies in 50 female rats treated with cyclosporin A (CsA, 40 mg·kg body wt-1·48 h-1) for either 10, 20, or 30 days (groups CsA 10, CsA 20, and CsA 30, respectively); control rats received olive oil (group N). Body weight gain, sodium metabolism, and plasma volume were not altered by CsA administration in any group. GFR was decreased in group CsA 10 vs. group N (-10%, P <0.05) and was further impaired in groups CsA 20 and CsA 30 (-45%, P <0.01 vs. group N). RM studies showed a significant decrease of single-nephron glomerular filtration rate (SNGFR) in group CsA 30 vs. group N (-26%, P <0.01) after the fall of glomerular capillary pressure (Pgc; -8%, P <0.05), the increase of afferent arteriole resistance (Ra; +40%, P <0.05), and the consequent decrease of glomerular plasma flow (GPF; -28%, P <0.05). Thirty days after CsA withdrawal, SNGFR returned to normal values (P <0.01 vs. group CsA) as a result of the normalization of Pgc, Ra, and GPF. EM showed only a progressive vacuolation of proximal and distal tubular cells. These data suggest that medium-term administration of CsA is associated with reversible changes in glomerular dynamics and only mild histological lesions.
KW - Micropuncture
KW - Nephrotoxicity
UR - http://www.scopus.com/inward/record.url?scp=0025827435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025827435&partnerID=8YFLogxK
M3 - Article
VL - 260
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 6 29-6
ER -