MEF promotes stemness in the pathogenesis of gliomas

Elena Bazzoli; Teodoro Pulvirenti; Moritz C. Oberstadt; Fabiana Perna; Boyoung Wee; Nikolaus Schultz; Jason T. Huse; Elena I. Fomchenko; Francesca Voza; Viviane Tabar; Cameron W. Brennan; Lisa M. Deangelis; Stephen D. Nimer; Eric C. Holland; Massimo Squatrito

doi:10.1016/j.stem.2012.09.012

MEF promotes stemness in the pathogenesis of gliomas

Elena Bazzoli, Teodoro Pulvirenti, Moritz C. Oberstadt, Fabiana Perna, Boyoung Wee, Nikolaus Schultz, Jason T. Huse, Elena I. Fomchenko, Francesca Voza, Viviane Tabar, Cameron W. Brennan, Lisa M. Deangelis, Stephen D. Nimer, Eric C. Holland, Massimo Squatrito

Centro San Giovanni di Dio - Fatebenefratelli

Research output: Contribution to journal › Article › peer-review

Abstract

High-grade gliomas are aggressive and uniformly fatal tumors, composed of a heterogeneous population of cells that include many with stem-cell-like properties. The acquisition of stem-like traits might contribute to glioma initiation, growth, and recurrence. Here we investigated the role of the transcription factor myeloid Elf-1 like factor (MEF, also known as ELF4) in gliomas. We found that MEF is highly expressed in both human and mouse glioblastomas and its absence impairs gliomagenesis in a PDGF-driven glioma mouse model. We show that modulation of MEF levels in both mouse neural stem cells and human glioblastoma cells has a significant impact on neurosphere formation. Moreover, we identify Sox2 as a direct downstream target of MEF. Taken together, our studies implicate MEF as a previously unrecognized gatekeeper gene in gliomagenesis that promotes stem cell characteristics through Sox2 activation.

Original language	English
Pages (from-to)	836-844
Number of pages	9
Journal	Cell Stem Cell
Volume	11
Issue number	6
DOIs	https://doi.org/10.1016/j.stem.2012.09.012
Publication status	Published - Dec 7 2012

ASJC Scopus subject areas

Cell Biology
Molecular Medicine
Genetics

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10.1016/j.stem.2012.09.012

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MEF promotes stemness in the pathogenesis of gliomas. / Bazzoli, Elena; Pulvirenti, Teodoro; Oberstadt, Moritz C.; Perna, Fabiana; Wee, Boyoung; Schultz, Nikolaus; Huse, Jason T.; Fomchenko, Elena I.; Voza, Francesca; Tabar, Viviane; Brennan, Cameron W.; Deangelis, Lisa M.; Nimer, Stephen D.; Holland, Eric C.; Squatrito, Massimo.

In: Cell Stem Cell, Vol. 11, No. 6, 07.12.2012, p. 836-844.

Research output: Contribution to journal › Article › peer-review

Bazzoli, Elena ; Pulvirenti, Teodoro ; Oberstadt, Moritz C. ; Perna, Fabiana ; Wee, Boyoung ; Schultz, Nikolaus ; Huse, Jason T. ; Fomchenko, Elena I. ; Voza, Francesca ; Tabar, Viviane ; Brennan, Cameron W. ; Deangelis, Lisa M. ; Nimer, Stephen D. ; Holland, Eric C. ; Squatrito, Massimo. / MEF promotes stemness in the pathogenesis of gliomas. In: Cell Stem Cell. 2012 ; Vol. 11, No. 6. pp. 836-844.

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title = "MEF promotes stemness in the pathogenesis of gliomas",

abstract = "High-grade gliomas are aggressive and uniformly fatal tumors, composed of a heterogeneous population of cells that include many with stem-cell-like properties. The acquisition of stem-like traits might contribute to glioma initiation, growth, and recurrence. Here we investigated the role of the transcription factor myeloid Elf-1 like factor (MEF, also known as ELF4) in gliomas. We found that MEF is highly expressed in both human and mouse glioblastomas and its absence impairs gliomagenesis in a PDGF-driven glioma mouse model. We show that modulation of MEF levels in both mouse neural stem cells and human glioblastoma cells has a significant impact on neurosphere formation. Moreover, we identify Sox2 as a direct downstream target of MEF. Taken together, our studies implicate MEF as a previously unrecognized gatekeeper gene in gliomagenesis that promotes stem cell characteristics through Sox2 activation.",

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AU - Brennan, Cameron W.

AU - Deangelis, Lisa M.

AU - Nimer, Stephen D.

AU - Holland, Eric C.

AU - Squatrito, Massimo

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MEF promotes stemness in the pathogenesis of gliomas

Abstract

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