MEF2 is a converging hub for histone deacetylase 4 and phosphatidylinositol 3-kinase/akt-induced transformation

Eros Di Giorgio, Andrea Clocchiatti, Sara Piccinin, Andrea Sgorbissa, Giulia Viviani, Paolo Peruzzo, Salvatore Romeo, Sabrina Rossi, Angelo Paolo Dei Tos, Roberta Maestro, Claudio Brancolini

Research output: Contribution to journalArticlepeer-review

Abstract

The MEF2-class IIa histone deacetylase (HDAC) axis operates in several differentiation pathways and in numerous adaptive responses. We show here that nuclear active HDAC4 and HDAC7 display transforming capability. HDAC4 oncogenic potential depends on the repression of a limited set of genes, most of which are MEF2 targets. Genes verified as targets of the MEF2-HDAC axis are also under the influence of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway that affects MEF2 protein stability. A signature of MEF2 target genes identified by this study is recurrently repressed in soft tissue sarcomas. Correlation studies depicted two distinct groups of soft tissue sarcomas: one in which MEF2 repression correlates with PTEN downregulation and a second group in which MEF2 repression correlates with HDAC4 levels. Finally, simultaneous pharmacological inhibition of the PI3K/Akt pathway and of MEF2-HDAC interaction shows additive effects on the transcription of MEF2 target genes and on sarcoma cells proliferation. Overall, our work pinpoints an important role of the MEF2-HDAC class IIa axis in tumorigenesis.

Original languageEnglish
Pages (from-to)4473-4491
Number of pages19
JournalMolecular and Cellular Biology
Volume33
Issue number22
DOIs
Publication statusPublished - Nov 15 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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