Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures: action of Tamm-Horsfall glycoprotein

Gian Paolo Bagnara; Luisa Valvassori; Marina Marini; Claudio Franceschi; Franca Serafini-Cessi

doi:10.1016/0045-6039(84)90016-2

Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures: action of Tamm-Horsfall glycoprotein

Gian Paolo Bagnara, Luisa Valvassori, Marina Marini, Claudio Franceschi, Franca Serafini-Cessi

Istituto Scienze Neurologiche

Research output: Contribution to journal › Article › peer-review

Abstract

Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.

Original language	English
Pages (from-to)	277-285
Number of pages	9
Journal	Cell Differentiation
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/0045-6039(84)90016-2
Publication status	Published - 1984

Keywords

bone marrow cultures
cell to cell interactions
glucides
hemopoietic microenvironment
megakaryocytopoiesis
Tamm-Horsfall glycoprotein

ASJC Scopus subject areas

Developmental Biology

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title = "Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures: action of Tamm-Horsfall glycoprotein",

abstract = "Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.",

keywords = "bone marrow cultures, cell to cell interactions, glucides, hemopoietic microenvironment, megakaryocytopoiesis, Tamm-Horsfall glycoprotein",

author = "Bagnara, {Gian Paolo} and Luisa Valvassori and Marina Marini and Claudio Franceschi and Franca Serafini-Cessi",

year = "1984",

doi = "10.1016/0045-6039(84)90016-2",

language = "English",

volume = "14",

pages = "277--285",

journal = "Cell Differentiation",

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TY - JOUR

T1 - Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures

T2 - action of Tamm-Horsfall glycoprotein

AU - Bagnara, Gian Paolo

AU - Valvassori, Luisa

AU - Marini, Marina

AU - Franceschi, Claudio

AU - Serafini-Cessi, Franca

PY - 1984

Y1 - 1984

N2 - Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.

AB - Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.

KW - bone marrow cultures

KW - cell to cell interactions

KW - glucides

KW - hemopoietic microenvironment

KW - megakaryocytopoiesis

KW - Tamm-Horsfall glycoprotein

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JO - Cell Differentiation

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ER -

Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures: action of Tamm-Horsfall glycoprotein

Abstract

Keywords

ASJC Scopus subject areas

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