Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures: action of Tamm-Horsfall glycoprotein

Gian Paolo Bagnara, Luisa Valvassori, Marina Marini, Claudio Franceschi, Franca Serafini-Cessi

Research output: Contribution to journalArticlepeer-review

Abstract

Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalCell Differentiation
Volume14
Issue number4
DOIs
Publication statusPublished - 1984

Keywords

  • bone marrow cultures
  • cell to cell interactions
  • glucides
  • hemopoietic microenvironment
  • megakaryocytopoiesis
  • Tamm-Horsfall glycoprotein

ASJC Scopus subject areas

  • Developmental Biology

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