TY - JOUR
T1 - Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures
T2 - action of Tamm-Horsfall glycoprotein
AU - Bagnara, Gian Paolo
AU - Valvassori, Luisa
AU - Marini, Marina
AU - Franceschi, Claudio
AU - Serafini-Cessi, Franca
PY - 1984
Y1 - 1984
N2 - Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.
AB - Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.
KW - bone marrow cultures
KW - cell to cell interactions
KW - glucides
KW - hemopoietic microenvironment
KW - megakaryocytopoiesis
KW - Tamm-Horsfall glycoprotein
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U2 - 10.1016/0045-6039(84)90016-2
DO - 10.1016/0045-6039(84)90016-2
M3 - Article
C2 - 6509554
AN - SCOPUS:0021746253
VL - 14
SP - 277
EP - 285
JO - Cell Differentiation
JF - Cell Differentiation
SN - 0045-6039
IS - 4
ER -