Abstract
Degradation of extracellular matrix components is central to many pathological features of chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross-linked amino acids whose urine levels are considered representative of elastin breakdown. The aim of this study was to apply a novel methodology, based on high-performance capillary electrophoresis, to the quantification of desmosine and isodesmosine in 11 patients with stable chronic obstructive pulmonary disease (COPD), 10 with an exacerbation of COPD, nine with α1-antitrypsin deficiency, 13 with bronchiectasis, and 11 adults with cystic fibrosis, in comparison to 24 controls. It was found that, in patients with stable COPD, urinary desmosine levels were higher than in controls (p=0.03), but lower than in COPD subjects with an exacerbation (p≤0.05). The highest desmosine levels were found in subjects with α1-antitrypsin deficiency, bronchiectasis and cystic fibrosis (p1-antitrypsin deficiency suggest that this method might he used to evaluate the putative efficacy of replacement therapy. (C) ERS Journals Ltd 2000.
| Original language | English |
|---|---|
| Pages (from-to) | 1039-1045 |
| Number of pages | 7 |
| Journal | European Respiratory Journal |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- α-Antitrypsin deficiency
- Bronchiectasis
- Chronic obstructive pulmonary disease
- Cystic fibrosis
- Lung elastin
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients. / Viglio, S.; Iadarola, P.; Lupi, A.; Trisolini, R.; Tinelli, C.; Balbi, B.; Grassi, V.; Worlitzsch, D.; Döring, G.; Meloni, F.; Meyer, K. C.; Dowson, L.; Hill, S. L.; Stockley, R. A.; Luisetti, M.
In: European Respiratory Journal, Vol. 15, No. 6, 2000, p. 1039-1045.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients
AU - Viglio, S.
AU - Iadarola, P.
AU - Lupi, A.
AU - Trisolini, R.
AU - Tinelli, C.
AU - Balbi, B.
AU - Grassi, V.
AU - Worlitzsch, D.
AU - Döring, G.
AU - Meloni, F.
AU - Meyer, K. C.
AU - Dowson, L.
AU - Hill, S. L.
AU - Stockley, R. A.
AU - Luisetti, M.
PY - 2000
Y1 - 2000
N2 - Degradation of extracellular matrix components is central to many pathological features of chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross-linked amino acids whose urine levels are considered representative of elastin breakdown. The aim of this study was to apply a novel methodology, based on high-performance capillary electrophoresis, to the quantification of desmosine and isodesmosine in 11 patients with stable chronic obstructive pulmonary disease (COPD), 10 with an exacerbation of COPD, nine with α1-antitrypsin deficiency, 13 with bronchiectasis, and 11 adults with cystic fibrosis, in comparison to 24 controls. It was found that, in patients with stable COPD, urinary desmosine levels were higher than in controls (p=0.03), but lower than in COPD subjects with an exacerbation (p≤0.05). The highest desmosine levels were found in subjects with α1-antitrypsin deficiency, bronchiectasis and cystic fibrosis (p1-antitrypsin deficiency suggest that this method might he used to evaluate the putative efficacy of replacement therapy. (C) ERS Journals Ltd 2000.
AB - Degradation of extracellular matrix components is central to many pathological features of chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross-linked amino acids whose urine levels are considered representative of elastin breakdown. The aim of this study was to apply a novel methodology, based on high-performance capillary electrophoresis, to the quantification of desmosine and isodesmosine in 11 patients with stable chronic obstructive pulmonary disease (COPD), 10 with an exacerbation of COPD, nine with α1-antitrypsin deficiency, 13 with bronchiectasis, and 11 adults with cystic fibrosis, in comparison to 24 controls. It was found that, in patients with stable COPD, urinary desmosine levels were higher than in controls (p=0.03), but lower than in COPD subjects with an exacerbation (p≤0.05). The highest desmosine levels were found in subjects with α1-antitrypsin deficiency, bronchiectasis and cystic fibrosis (p1-antitrypsin deficiency suggest that this method might he used to evaluate the putative efficacy of replacement therapy. (C) ERS Journals Ltd 2000.
KW - α-Antitrypsin deficiency
KW - Bronchiectasis
KW - Chronic obstructive pulmonary disease
KW - Cystic fibrosis
KW - Lung elastin
UR - http://www.scopus.com/inward/record.url?scp=0034123654&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034123654&partnerID=8YFLogxK
U2 - 10.1034/j.1399-3003.2000.01511.x
DO - 10.1034/j.1399-3003.2000.01511.x
M3 - Article
C2 - 10885422
AN - SCOPUS:0034123654
VL - 15
SP - 1039
EP - 1045
JO - European Journal of Respiratory Diseases
JF - European Journal of Respiratory Diseases
SN - 0903-1936
IS - 6
ER -