Melanoma cell lysis by human CTL clones

Differential involvement of T3, T8 and HLA antigens

G. Fossati, A. Anichini, G. Parmiani

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Three lymphocyte clones, derived by micromanipulation from peripheral blood lymphocytes (PBL) of a melanoma patient and expressing a broad pattern of reactivity against different target cells, were analyzed for the involvement of T-cell markers and HLA antigens in the lysis of target cells by blocking experiments with a panel of monoclonal antibodies (MAbs). The clones lysed autologous melanoma cells (Me 28) and 18 out of 22 allogeneic targets including neoplastic and normal cells of different histological origin. Anti-T3 and anti-T8 MAbs strongly inhibited the cytotoxicity of the lymphocyte clones against Me 28, 3 allogeneic melanomas and 3 carcinomas, but failed to affect the lysis of K562. Anti-HLA class-I MAb (w6/32) produced a significant enhancement of the lysis of Me 28 by the 3 clones without modifying cytotoxicity against one allogeneic melanoma or against K562 cells. Anti-HLA class-II MAb (D1.12) did not affect the lysis of the same targets by the 3 clones. These results thus indicate that some anti-melanoma CTL clones may interact with autologous tumor cells by the T3 and T8 structures in an HLA class-I unrestricted manner.

Original languageEnglish
Pages (from-to)689-694
Number of pages6
JournalInternational Journal of Cancer
Volume39
Issue number6
Publication statusPublished - 1987

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CD3 Antigens
HLA Antigens
Melanoma
Clone Cells
Lymphocytes
Monoclonal Antibodies
Micromanipulation
CD8-Positive T-Lymphocytes
K562 Cells
Differentiation Antigens
Carcinoma
T-Lymphocytes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Melanoma cell lysis by human CTL clones : Differential involvement of T3, T8 and HLA antigens. / Fossati, G.; Anichini, A.; Parmiani, G.

In: International Journal of Cancer, Vol. 39, No. 6, 1987, p. 689-694.

Research output: Contribution to journalArticle

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