MELAS mitochondrial DNA mutation A3243G reduces glutamate transport in cybrids cell lines

Jacopo C. DiFrancesco, J. Mark Cooper, Amanda Lam, Paul E. Hart, Lucio Tremolizzo, Carlo Ferrarese, Antony H. Schapira

Research output: Contribution to journalArticlepeer-review

Abstract

MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes) is commonly associated with the A3243G mitochondrial DNA (mtDNA) mutation encoding the transfer RNA of leucine (UUR) (tRNA Leu(UUR)). The pathogenetic mechanisms of this mutation are not completely understood. Neuronal functions are particularly vulnerable to alterations in oxidative phosphorylation, which may affect the function of the neurotransmitter glutamate, leading to excitotoxicity. In order to investigate the possible effects of A3243G upon glutamate homeostasis, we assessed glutamate uptake in osteosarcoma-derived cytoplasmic hybrids (cybrids) expressing high levels of this mutation. High-affinity Na+-dependent glutamate uptake was assessed as radioactive [3H]-glutamate influx mediated by specific excitatory amino acid transporters (EAATs). The maximal rate (Vmax) of Na+-dependent glutamate uptake was significantly reduced in all the mutant clones. Although the defect did not relate to either the mutant load or magnitude of oxidative phosphorylation defect, we found an inverse relationship between A3243G mutation load and mitochondrial ATP synthesis, without any evidence of increased cellular or mitochondrial free radical production in these A3243G clones. These data suggest that a defect of glutamate transport in MELAS neurons may be due to decreased energy production and might be involved in mediating the pathogenic effects of the A3243G mtDNA mutation.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalExperimental Neurology
Volume212
Issue number1
DOIs
Publication statusPublished - Jul 2008

Keywords

  • A3243G
  • Cybrid
  • Excitotoxicity
  • Glutamate
  • MELAS
  • Mitochondria

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

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