Melatonin increases the intensity of respiratory burst and prevents L-selectin shedding in human neutrophils in vitro

Rina Recchioni, Fiorella Marcheselli, Fausto Moroni, Rezsö Gáspár, Sándor Damjanovich, Carlo Pieri

Research output: Contribution to journalArticle

Abstract

Effects of melatonin priming of neutrophils and subsequent increase of phorbol 12-miristate 13-acetate stimulated respiratory burst were investigated on the modulation of L-selectin shedding and MAC-1 upregulation. Respiratory burst related H2O2 production and adhesion molecule expression were quantified by flow cytometry. Phorbol 12-miristate 13-acetate dose dependence of intracellular oxidation and adhesion molecule expression showed no relationship between respiratory burst intensity and MAC-1 expression or L-selectin shedding. Treatment of cells with 12.5 nM phorbol 12-miristate 13-acetate resulted in less than 20% of the respiratory burst response, however it induced 91.7% of total MAC-1 expression and 62.8% of L-selectin shedding. Melatonin priming experiments showed also no connection between the extent of respiratory burst and MAC-1 expression, however melatonin priming almost completely prevented L-selectin down-regulation elicited by phorbol 12-miristate 13-acetate, without affecting MAC-1 expression. It is suggested that melatonin may inhibit metalloproteases responsible for L-selectin cleavage.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number1
DOIs
Publication statusPublished - Nov 9 1998

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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