Melatonin increases the intensity of respiratory burst and prevents L-selectin shedding in human neutrophils in vitro

Rina Recchioni, Fiorella Marcheselli, Fausto Moroni, Rezsö Gáspár, Sándor Damjanovich, Carlo Pieri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Effects of melatonin priming of neutrophils and subsequent increase of phorbol 12-miristate 13-acetate stimulated respiratory burst were investigated on the modulation of L-selectin shedding and MAC-1 upregulation. Respiratory burst related H2O2 production and adhesion molecule expression were quantified by flow cytometry. Phorbol 12-miristate 13-acetate dose dependence of intracellular oxidation and adhesion molecule expression showed no relationship between respiratory burst intensity and MAC-1 expression or L-selectin shedding. Treatment of cells with 12.5 nM phorbol 12-miristate 13-acetate resulted in less than 20% of the respiratory burst response, however it induced 91.7% of total MAC-1 expression and 62.8% of L-selectin shedding. Melatonin priming experiments showed also no connection between the extent of respiratory burst and MAC-1 expression, however melatonin priming almost completely prevented L-selectin down-regulation elicited by phorbol 12-miristate 13-acetate, without affecting MAC-1 expression. It is suggested that melatonin may inhibit metalloproteases responsible for L-selectin cleavage.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number1
DOIs
Publication statusPublished - Nov 9 1998

Fingerprint

L-Selectin
Respiratory Burst
Melatonin
Neutrophils
Acetates
Adhesion
Molecules
Flow cytometry
Metalloproteases
Flow Cytometry
Up-Regulation
Down-Regulation
Cells
Modulation
In Vitro Techniques
Oxidation
phorbol
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Melatonin increases the intensity of respiratory burst and prevents L-selectin shedding in human neutrophils in vitro. / Recchioni, Rina; Marcheselli, Fiorella; Moroni, Fausto; Gáspár, Rezsö; Damjanovich, Sándor; Pieri, Carlo.

In: Biochemical and Biophysical Research Communications, Vol. 252, No. 1, 09.11.1998, p. 20-24.

Research output: Contribution to journalArticle

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