Melatonin provokes cell death in human B-lymphoma cells by mitochondrial-dependent apoptotic pathway activation

Oriana Trubiani, Rina Recchioni, Fausto Moroni, Jacopo Pizzicannella, Sergio Caputi, Roberto Di Primio

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Apoptosis is an important cell suicide programme involved in physiological and pathological processes. Apoptosis can be induced in different ways depending on cell type and acquired signal. Melatonin, the major secretory product of the pineal gland, participates in many important physiological functions and displays a remarkable functional versatility exhibiting antioxidant, oncostatic, anti-aging, and immunomodulatory properties. Recently, it has been shown that, in addition to pineal gland, human lymphoid cells are an important physiological source of melatonin and that may be involved in the regulation of the immune system. In this work, we examine the effect of melatonin on RAMOS-1 human leukaemic cells. Cell growth and viability, DNA fragmentation and JC-1, and annexin V expression have been determined. To elucidate the mechanism of action of melatonin, Western blot analyses for Bcl-2 and caspase-3 expression, and cytochrome c release were carried out. The results suggest that the apoptotic effect of melatonin is associated with cell-cycle arrest, downregulation of Bcl-2, mitochondrial membrane depolarization, cytochrome c release and activation of caspase-3. The intrinsic (mitochondrial dependent) pathway of caspase activation is the 'point of no return' commitment to cell death. Taken together, our study indicates that melatonin may play a role as potential therapeutic drug in specific lymphoproliferative diseases.

Original languageEnglish
Pages (from-to)425-431
Number of pages7
JournalJournal of Pineal Research
Volume39
Issue number4
DOIs
Publication statusPublished - Nov 2005

Fingerprint

B-Cell Lymphoma
Melatonin
Cell Death
Pineal Gland
Cytochromes c
Caspase 3
Apoptosis
Physiological Phenomena
Annexin A5
Mitochondrial Membranes
DNA Fragmentation
Pathologic Processes
Caspases
Cell Cycle Checkpoints
Suicide
Immune System
Cell Survival
Down-Regulation
Antioxidants
Western Blotting

Keywords

  • Apoptosis
  • Caspase-3
  • Cytochrome c
  • Lymphoma
  • Melatonin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Melatonin provokes cell death in human B-lymphoma cells by mitochondrial-dependent apoptotic pathway activation. / Trubiani, Oriana; Recchioni, Rina; Moroni, Fausto; Pizzicannella, Jacopo; Caputi, Sergio; Di Primio, Roberto.

In: Journal of Pineal Research, Vol. 39, No. 4, 11.2005, p. 425-431.

Research output: Contribution to journalArticle

Trubiani, Oriana ; Recchioni, Rina ; Moroni, Fausto ; Pizzicannella, Jacopo ; Caputi, Sergio ; Di Primio, Roberto. / Melatonin provokes cell death in human B-lymphoma cells by mitochondrial-dependent apoptotic pathway activation. In: Journal of Pineal Research. 2005 ; Vol. 39, No. 4. pp. 425-431.
@article{0a6633802dc3439281d4c146ca5ea088,
title = "Melatonin provokes cell death in human B-lymphoma cells by mitochondrial-dependent apoptotic pathway activation",
abstract = "Apoptosis is an important cell suicide programme involved in physiological and pathological processes. Apoptosis can be induced in different ways depending on cell type and acquired signal. Melatonin, the major secretory product of the pineal gland, participates in many important physiological functions and displays a remarkable functional versatility exhibiting antioxidant, oncostatic, anti-aging, and immunomodulatory properties. Recently, it has been shown that, in addition to pineal gland, human lymphoid cells are an important physiological source of melatonin and that may be involved in the regulation of the immune system. In this work, we examine the effect of melatonin on RAMOS-1 human leukaemic cells. Cell growth and viability, DNA fragmentation and JC-1, and annexin V expression have been determined. To elucidate the mechanism of action of melatonin, Western blot analyses for Bcl-2 and caspase-3 expression, and cytochrome c release were carried out. The results suggest that the apoptotic effect of melatonin is associated with cell-cycle arrest, downregulation of Bcl-2, mitochondrial membrane depolarization, cytochrome c release and activation of caspase-3. The intrinsic (mitochondrial dependent) pathway of caspase activation is the 'point of no return' commitment to cell death. Taken together, our study indicates that melatonin may play a role as potential therapeutic drug in specific lymphoproliferative diseases.",
keywords = "Apoptosis, Caspase-3, Cytochrome c, Lymphoma, Melatonin",
author = "Oriana Trubiani and Rina Recchioni and Fausto Moroni and Jacopo Pizzicannella and Sergio Caputi and {Di Primio}, Roberto",
year = "2005",
month = "11",
doi = "10.1111/j.1600-079X.2005.00270.x",
language = "English",
volume = "39",
pages = "425--431",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Melatonin provokes cell death in human B-lymphoma cells by mitochondrial-dependent apoptotic pathway activation

AU - Trubiani, Oriana

AU - Recchioni, Rina

AU - Moroni, Fausto

AU - Pizzicannella, Jacopo

AU - Caputi, Sergio

AU - Di Primio, Roberto

PY - 2005/11

Y1 - 2005/11

N2 - Apoptosis is an important cell suicide programme involved in physiological and pathological processes. Apoptosis can be induced in different ways depending on cell type and acquired signal. Melatonin, the major secretory product of the pineal gland, participates in many important physiological functions and displays a remarkable functional versatility exhibiting antioxidant, oncostatic, anti-aging, and immunomodulatory properties. Recently, it has been shown that, in addition to pineal gland, human lymphoid cells are an important physiological source of melatonin and that may be involved in the regulation of the immune system. In this work, we examine the effect of melatonin on RAMOS-1 human leukaemic cells. Cell growth and viability, DNA fragmentation and JC-1, and annexin V expression have been determined. To elucidate the mechanism of action of melatonin, Western blot analyses for Bcl-2 and caspase-3 expression, and cytochrome c release were carried out. The results suggest that the apoptotic effect of melatonin is associated with cell-cycle arrest, downregulation of Bcl-2, mitochondrial membrane depolarization, cytochrome c release and activation of caspase-3. The intrinsic (mitochondrial dependent) pathway of caspase activation is the 'point of no return' commitment to cell death. Taken together, our study indicates that melatonin may play a role as potential therapeutic drug in specific lymphoproliferative diseases.

AB - Apoptosis is an important cell suicide programme involved in physiological and pathological processes. Apoptosis can be induced in different ways depending on cell type and acquired signal. Melatonin, the major secretory product of the pineal gland, participates in many important physiological functions and displays a remarkable functional versatility exhibiting antioxidant, oncostatic, anti-aging, and immunomodulatory properties. Recently, it has been shown that, in addition to pineal gland, human lymphoid cells are an important physiological source of melatonin and that may be involved in the regulation of the immune system. In this work, we examine the effect of melatonin on RAMOS-1 human leukaemic cells. Cell growth and viability, DNA fragmentation and JC-1, and annexin V expression have been determined. To elucidate the mechanism of action of melatonin, Western blot analyses for Bcl-2 and caspase-3 expression, and cytochrome c release were carried out. The results suggest that the apoptotic effect of melatonin is associated with cell-cycle arrest, downregulation of Bcl-2, mitochondrial membrane depolarization, cytochrome c release and activation of caspase-3. The intrinsic (mitochondrial dependent) pathway of caspase activation is the 'point of no return' commitment to cell death. Taken together, our study indicates that melatonin may play a role as potential therapeutic drug in specific lymphoproliferative diseases.

KW - Apoptosis

KW - Caspase-3

KW - Cytochrome c

KW - Lymphoma

KW - Melatonin

UR - http://www.scopus.com/inward/record.url?scp=27744592273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744592273&partnerID=8YFLogxK

U2 - 10.1111/j.1600-079X.2005.00270.x

DO - 10.1111/j.1600-079X.2005.00270.x

M3 - Article

C2 - 16207299

AN - SCOPUS:27744592273

VL - 39

SP - 425

EP - 431

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 4

ER -