Melatonin regulates matrix metalloproteinases after traumatic experimental spinal cord injury

Emanuela Esposito, Tiziana Genovese, Rocco Caminiti, Placido Bramanti, Rosaria Meli, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The matrix metalloproteinases (MMPs) are important enzymes that regulate developmental processes, maintain normal physiology in adulthood and have reparative roles at specific stages after an insult to the nervous system. MMPs, particularly MMP-9/gelatinase B, promote early inflammation and barrier disruption after spinal cord injury (SCI). Recently, we have reported that the pineal secretory product melatonin exerts important anti-inflammatory effects in an experimental model of SCI induced by the application of vascular clips (force of 24 g) to the dura after a four-level T5-T8 laminectomy. However, no reports are available on the relationship between the activity of MMPs and melatonin's anti-inflammatory effects. The aim of the present study was to evaluate whether the protective effect of melatonin observed in SCI is related to the regulation of MMP-9 and MMP-2 in mice. Biochemical and zymographic methods were used to analyze MMP-9 and -2 expression and activities in spinal cord tissue from SCI-treated mice at 24 hr after the trauma. Our studies reveal that melatonin reduced SCI and lipid peroxidation in spinal cord at 24 hr after SCI. Melatonin also diminished proMMP-9 and -2 activities that were induced in the spinal cord tissues at 24 hr after SCI. The reduced activities of MMP-9 and -2 were associated with depressed expression of TNF-α. We propose that melatonin's ability to reduce SCI in mice is also related to a reduction in MMP-9 and MMP-2 activity and expression.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalJournal of Pineal Research
Volume45
Issue number2
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Melatonin
Matrix Metalloproteinases
Spinal Cord Injuries
Matrix Metalloproteinase 9
Matrix Metalloproteinase 2
Spinal Cord
Anti-Inflammatory Agents
Laminectomy
Surgical Instruments
Nervous System
Lipid Peroxidation
Blood Vessels
Theoretical Models
Inflammation
Wounds and Injuries
Enzymes

Keywords

  • Free radicals
  • Inflammation
  • Metalloproteinase
  • Spinal cord damage
  • Spinal cord injury

ASJC Scopus subject areas

  • Endocrinology

Cite this

Melatonin regulates matrix metalloproteinases after traumatic experimental spinal cord injury. / Esposito, Emanuela; Genovese, Tiziana; Caminiti, Rocco; Bramanti, Placido; Meli, Rosaria; Cuzzocrea, Salvatore.

In: Journal of Pineal Research, Vol. 45, No. 2, 09.2008, p. 149-156.

Research output: Contribution to journalArticle

Esposito, Emanuela ; Genovese, Tiziana ; Caminiti, Rocco ; Bramanti, Placido ; Meli, Rosaria ; Cuzzocrea, Salvatore. / Melatonin regulates matrix metalloproteinases after traumatic experimental spinal cord injury. In: Journal of Pineal Research. 2008 ; Vol. 45, No. 2. pp. 149-156.
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