Membrane lipid alterations as a possible basis for melanocyte degeneration in vitiligo

Maria Lucia Dell'Anna, Monica Ottaviani, Veronica Albanesi, Andrea Paro Vidolin, Giovanni Leone, Carmela Ferraro, Andrea Cossarizza, Luisa Rossi, Mauro Picardo

Research output: Contribution to journalArticle

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Abstract

The occurrence of oxidative stress has been proposed as a pathogenetic mechanism for melanocyte degeneration in vitiligo. In order to evaluate this possible correlation we focused on the lipid component of cell membranes. We observed in vitiligo melanocytes, through FACS methods, an increased median fluorescence intensity of rhodamine 123 and C11-BODIPY581/591 indicating a spontaneous higher production of reactive oxygen species (ROS) and membrane lipoperoxidation, associated with an altered pattern of cardiolipin (CL) distribution, defined on the basis of the fluorescence pattern after staining with 10-nonyl acridine orange. We confirmed membrane peroxidation by confocal and contrast-phase microscopes and demonstrated impaired activity of the mitochondrial electron transport chain (ETC) complex I. Finally, we observed increased apoptotic events following exposure to the pro-oxidant cumene hydroperoxide by Annexin V/propidium iodide fluorescence. We hypothesize that in vitiligo melanocytes lipid instability, with a defect in the synthesis or recycling of CL, induces ETC impairment and ROS production. In basal conditions melanocytes maintain the redox balance whereas following chemical or physical stress ROS-mediated membrane peroxidation is increased with a possible further CL oxidation, leading to cell death or detachment.

Original languageEnglish
Pages (from-to)1226-1233
Number of pages8
JournalJournal of Investigative Dermatology
Volume127
Issue number5
DOIs
Publication statusPublished - May 2007

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Vitiligo
Melanocytes
Membrane Lipids
Cardiolipins
Reactive Oxygen Species
Fluorescence
Membranes
Rhodamine 123
Electron Transport Complex I
Lipids
Acridine Orange
Oxidative stress
Propidium
Annexin A5
Recycling
Cell death
Cell membranes
Electron Transport
Oxidation-Reduction
Oxidative Stress

ASJC Scopus subject areas

  • Dermatology

Cite this

Membrane lipid alterations as a possible basis for melanocyte degeneration in vitiligo. / Dell'Anna, Maria Lucia; Ottaviani, Monica; Albanesi, Veronica; Vidolin, Andrea Paro; Leone, Giovanni; Ferraro, Carmela; Cossarizza, Andrea; Rossi, Luisa; Picardo, Mauro.

In: Journal of Investigative Dermatology, Vol. 127, No. 5, 05.2007, p. 1226-1233.

Research output: Contribution to journalArticle

Dell'Anna, Maria Lucia ; Ottaviani, Monica ; Albanesi, Veronica ; Vidolin, Andrea Paro ; Leone, Giovanni ; Ferraro, Carmela ; Cossarizza, Andrea ; Rossi, Luisa ; Picardo, Mauro. / Membrane lipid alterations as a possible basis for melanocyte degeneration in vitiligo. In: Journal of Investigative Dermatology. 2007 ; Vol. 127, No. 5. pp. 1226-1233.
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