Membrane transfer from tumor cells overcomes deficient phagocytic ability of plasmacytoid dendritic cells for the acquisition and presentation of tumor antigens

Irene Bonaccorsi, Barbara Morandi, Olga Antsiferova, Gregorio Costa, Daniela Oliveri, Romana Conte, Gaetana Pezzino, Giovanna Vermiglio, Giuseppe Pio Anastasi, Giuseppe Navarra, Christian Münz, Emma Di Carlo, Maria Cristina Mingari, Guido Ferlazzo

Research output: Contribution to journalArticle

Abstract

The potential contribution of plasmacytoid dendritic cells (pDCs) in the presentation of tumor cell Ags remains unclear, and some controversies exist with regard to the ability of pDCs to phagocytose cell-derived particulate Ags and cross-present them to MHC class I-restricted T lymphocytes. In this study, we show that human pDCs, although inefficient in the internalization of cell membrane fragments by phagocytosis, can efficiently acquire membrane patches and associated molecules from cancer cells of different histotypes. The transfer of membrane patches to pDCs occurred in a very short time and required cell-to-cell contact. Membrane transfer also included intact HLA complexes, and the acquired Ags could be efficiently recognized on pDCs by tumorspecific CD8+ T cells. Remarkably, pDCs isolated from human colon cancer tissues displayed a strong surface expression of epithelial cell adhesion molecule, indicating that the exchange of exogenous Ags between pDCs and tumor cells also can occur in vivo. These data demonstrate that pDCs are well suited to acquire membrane patches from contiguous tumor cells by a cell-tocell contact-dependent mechanism that closely resembles "trogocytosis." This phenomenon may allow pDCs to proficiently present tumor cell-derived Ags, despite limited properties of endophagocytosis.

Original languageEnglish
Pages (from-to)824-832
Number of pages9
JournalJournal of Immunology
Volume192
Issue number2
DOIs
Publication statusPublished - Jan 15 2014

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Neoplasm Antigens
Dendritic Cells
Membranes
Neoplasms
T-Lymphocytes
Cytophagocytosis
Phagocytosis
Colonic Neoplasms
Cell Membrane

ASJC Scopus subject areas

  • Immunology

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Membrane transfer from tumor cells overcomes deficient phagocytic ability of plasmacytoid dendritic cells for the acquisition and presentation of tumor antigens. / Bonaccorsi, Irene; Morandi, Barbara; Antsiferova, Olga; Costa, Gregorio; Oliveri, Daniela; Conte, Romana; Pezzino, Gaetana; Vermiglio, Giovanna; Anastasi, Giuseppe Pio; Navarra, Giuseppe; Münz, Christian; Carlo, Emma Di; Mingari, Maria Cristina; Ferlazzo, Guido.

In: Journal of Immunology, Vol. 192, No. 2, 15.01.2014, p. 824-832.

Research output: Contribution to journalArticle

Bonaccorsi, I, Morandi, B, Antsiferova, O, Costa, G, Oliveri, D, Conte, R, Pezzino, G, Vermiglio, G, Anastasi, GP, Navarra, G, Münz, C, Carlo, ED, Mingari, MC & Ferlazzo, G 2014, 'Membrane transfer from tumor cells overcomes deficient phagocytic ability of plasmacytoid dendritic cells for the acquisition and presentation of tumor antigens', Journal of Immunology, vol. 192, no. 2, pp. 824-832. https://doi.org/10.4049/jimmunol.1301039
Bonaccorsi, Irene ; Morandi, Barbara ; Antsiferova, Olga ; Costa, Gregorio ; Oliveri, Daniela ; Conte, Romana ; Pezzino, Gaetana ; Vermiglio, Giovanna ; Anastasi, Giuseppe Pio ; Navarra, Giuseppe ; Münz, Christian ; Carlo, Emma Di ; Mingari, Maria Cristina ; Ferlazzo, Guido. / Membrane transfer from tumor cells overcomes deficient phagocytic ability of plasmacytoid dendritic cells for the acquisition and presentation of tumor antigens. In: Journal of Immunology. 2014 ; Vol. 192, No. 2. pp. 824-832.
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