Membranes of activated CD4+ T cells expressing T cell receptor (TcR) αβ or TcR γδ induce IgE synthesis by human B cells in the presence of interleukin-4

Hugues Gascan, Gregorio G. Aversa, Jean François Gauchat, Peter Van Vlasselaer, Maria Grazia Roncarolo, Hans Yssel, Marilyn Kehry, Hergen Spits, Jan E. De Vries

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study it is demonstrated that human B cells can be induced to switch to IgE production following a contact-mediated signal provided by activated T cell receptor (TcR) γδ+, CD4+ and TcR αβ+, CD4+ T cell clones and interleukin (IL)-4. The signal provided by these T cell clones was antigen nonspecific, indicating that the TcR αβ/CD3 or TcR γδ/CD3 complexes were not involved in these T-B cell interactions. Activated TcR αβ+, CD8+, and TcR γδ+, CD4-CD8-, or resting CD4+ T cell clones were ineffective. Intact TcR αβ+ or TcR γδ+, CD4+ T cell clones could be replaced by plasma membrane-enriched fractions isolated from these activated CD4+ T cell clones. In contrast, membranes isolated from resting TcR αβ+, CD4+, TcR γδ+, CD4+ T cell clones or an Epstein-Barr virus (EBV)-transformed B cell line (EBV-LCL) failed to provide the costimulatory signal that, in addition to IL-4, is required for induction of IgE synthesis. As described for intact CD4+ T cells, CD4+ T cell membranes induced purified surface IgM+ B cells to switch to IgG4- and IgE- but not to IgA-producing cells, excluding the possibility of a preferential outgrowth of IgG4- and IgE-committed B cells. The membrane activity was inhibited by protease or heat treatment. Induction of IgE synthesis by B cells co-cultured with both TcR αβ+, CD4+ and TcR γδ+, CD4+ T cell clones and membrane preparations of these cells was blocked by anti-class II major histocompatibility complex (MHC) monoclonal antibodies (mAb), whereas various anti-CD4 mAb had differential blocking effects. Murine L cells, or EBV-LCL transfected with CD4 could not replace CD4+ T cell clones. These results indicate that, although CD4 and class II MHC antigens are required for productive CD4+ T cell clone-B cell interactions, an additional signal, provided by a membrane associated (glyco)protein that is induced by activation of both TcR αβ and TcR γδ, CD4+ T cells, is needed for induction of IgE production in the presence of IL-4.

Original languageEnglish
Pages (from-to)1133-1141
Number of pages9
JournalEuropean Journal of Immunology
Volume22
Issue number5
Publication statusPublished - May 1992

ASJC Scopus subject areas

  • Immunology

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