Merging colloidal nanoplasmonics and surface plasmon resonance spectroscopy for enhanced profiling of multiple myeloma-derived exosomes

Giuseppe Di Noto, Antonella Bugatti, Andrea Zendrini, Elena Laura Mazzoldi, Alessandro Montanelli, Luigi Caimi, Marco Rusnati, Doris Ricotta, Paolo Bergese

Research output: Contribution to journalArticlepeer-review

Abstract

A novel approach for sorting exosomes from multiple myeloma (MM), monoclonal gammopathy of undetermined significance (MGUS) and healthy individuals is presented. The method is based on the combination of colloidal gold nanoplasmonics and surface plasmon resonance (SPR) biosensing and probes distinctive colloidal properties of MM-derived exosomes, such as molar concentration and cell membrane binding preferences. It allowed to discover that MM patients produce about four folds more exosomes than MGUS and healthy individuals. In addition, it showed that among the analyzed exosomes, only the MM-derived ones bind heparin - a structural analog of heparan sulfate proteoglycans known to mediate exosome endocytosis - with an apparent dissociation constant (Kd) equal to about 1nM, indicating a high affinity binding. This plasmonic method complements the classical biochemical profiling approach to exosomes, expanding the MM biomarker panel and adding biosensors to the toolbox to diagnose MM. It may find applications for other diseases and has wider interest for fundamental and translational research involving exosomes.

Original languageEnglish
Pages (from-to)518-524
Number of pages7
JournalBiosensors and Bioelectronics
Volume77
DOIs
Publication statusPublished - Mar 15 2016

Keywords

  • Exosome
  • Multiple myeloma
  • Nanoplasmonics
  • Surface plasmon resonance spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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