Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms

Maria Sonia Sbarra; Antonella Di Poto; Enrica Saino; Livia Visai; Paolo Minzioni; Francesca Bragheri; Ilaria Cristiani

doi:10.1117/12.831889

Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms

Maria Sonia Sbarra, Antonella Di Poto, Enrica Saino, Livia Visai, Paolo Minzioni, Francesca Bragheri, Ilaria Cristiani

Istituti Clinici Scientifici Maugeri Spa – Società Benefit

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7373
DOIs	https://doi.org/10.1117/12.831889
Publication status	Published - 2009
Event	Therapeutic Laser Applications and Laser-Tissue Interactions IV - Munich, Germany Duration: Jun 17 2009 → Jun 18 2009

Other

Other	Therapeutic Laser Applications and Laser-Tissue Interactions IV
Country/Territory	Germany
City	Munich
Period	6/17/09 → 6/18/09

Keywords

Merocyanine 540 (MC 540)
Photodynamic therapy (PDT)
S. epidermidis biofilms

ASJC Scopus subject areas

Biomaterials
Electronic, Optical and Magnetic Materials
Radiology Nuclear Medicine and imaging
Atomic and Molecular Physics, and Optics

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10.1117/12.831889

Cite this

Sbarra, MS, Di Poto, A, Saino, E, Visai, L, Minzioni, P, Bragheri, F & Cristiani, I 2009, Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7373, 73730R, Therapeutic Laser Applications and Laser-Tissue Interactions IV, Munich, Germany, 6/17/09. https://doi.org/10.1117/12.831889

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abstract = "Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).",

keywords = "Merocyanine 540 (MC 540), Photodynamic therapy (PDT), S. epidermidis biofilms",

author = "Sbarra, {Maria Sonia} and {Di Poto}, Antonella and Enrica Saino and Livia Visai and Paolo Minzioni and Francesca Bragheri and Ilaria Cristiani",

year = "2009",

doi = "10.1117/12.831889",

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AU - Sbarra, Maria Sonia

AU - Di Poto, Antonella

AU - Saino, Enrica

AU - Visai, Livia

AU - Minzioni, Paolo

AU - Bragheri, Francesca

AU - Cristiani, Ilaria

PY - 2009

Y1 - 2009

N2 - Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).

AB - Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).

KW - Merocyanine 540 (MC 540)

KW - Photodynamic therapy (PDT)

KW - S. epidermidis biofilms

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DO - 10.1117/12.831889

M3 - Conference contribution

AN - SCOPUS:70349986202

SN - 9780819476494

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BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

T2 - Therapeutic Laser Applications and Laser-Tissue Interactions IV

Y2 - 17 June 2009 through 18 June 2009

ER -

Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms

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