Abstract
Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 7373 |
| DOIs | |
| Publication status | Published - 2009 |
| Event | Therapeutic Laser Applications and Laser-Tissue Interactions IV - Munich, Germany Duration: Jun 17 2009 → Jun 18 2009 |
Other
| Other | Therapeutic Laser Applications and Laser-Tissue Interactions IV |
|---|---|
| Country | Germany |
| City | Munich |
| Period | 6/17/09 → 6/18/09 |
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Keywords
- Merocyanine 540 (MC 540)
- Photodynamic therapy (PDT)
- S. epidermidis biofilms
ASJC Scopus subject areas
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Radiology Nuclear Medicine and imaging
- Atomic and Molecular Physics, and Optics
Cite this
Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms. / Sbarra, Maria Sonia; Di Poto, Antonella; Saino, Enrica; Visai, Livia; Minzioni, Paolo; Bragheri, Francesca; Cristiani, Ilaria.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7373 2009. 73730R.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Merocyanine-540 mediated photodynamic effects on Staphylococcus epidermidis biofilms
AU - Sbarra, Maria Sonia
AU - Di Poto, Antonella
AU - Saino, Enrica
AU - Visai, Livia
AU - Minzioni, Paolo
AU - Bragheri, Francesca
AU - Cristiani, Ilaria
PY - 2009
Y1 - 2009
N2 - Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).
AB - Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we evaluated the antimicrobial activity of merocyanine 540 (MC 540), a photosensitizing dye that is used for purging malignant cells from autologous bone marrow grafts, against Staphylococcus epidermidis biofilms. We evaluated the effect of the combined photodynamic action of MC 540 and 532 nm laser on the viability and structure of biofilms of two Staphylococcus epidermidis strains. Significant inactivation of cells was observed in the biofilms treated with MC-540 and then exposed to laser radiation. Furthermore we found that the PDT effect, on both types of cells, was significantly dependent on both the light-dose and on the impinging lightintensity. Disruption of PDT-treated biofilm was confirmed by scanning electron microscopy (SEM).
KW - Merocyanine 540 (MC 540)
KW - Photodynamic therapy (PDT)
KW - S. epidermidis biofilms
UR - http://www.scopus.com/inward/record.url?scp=70349986202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349986202&partnerID=8YFLogxK
U2 - 10.1117/12.831889
DO - 10.1117/12.831889
M3 - Conference contribution
AN - SCOPUS:70349986202
SN - 9780819476494
VL - 7373
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -