Mesenchymal stem cells and platelet-rich plasma enhance bone formation in sinus grafting: A histomorphometric study in minipigs

Francesco Pieri, Enrico Lucarelli, Giuseppe Corinaldesi, Giovanna Iezzi, Adriano Piattelli, Roberto Giardino, Massimo Bassi, Davide Donati, Claudio Marchetti

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue-engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone-to-implant contact (BIC) in maxillary sinus grafting. Material and Methods: Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis. Results: After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51% versus 18.98%; p=0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71% versus 6.63%; p=0.028). Conclusions: These findings show that sinus augmentation with MSCs-PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.

Original languageEnglish
Pages (from-to)539-546
Number of pages8
JournalJournal of Clinical Periodontology
Volume35
Issue number6
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Dental implants
  • Platelet-rich plasma
  • Sinus grafting
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Dentistry(all)

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