TY - JOUR
T1 - Mesenchymal stem cells expressing GD2 and CD271 correlate with breast cancer-initiating cells in bone marrow
AU - De Giorgi, Ugo
AU - Cohen, Evan N.
AU - Gao, Hui
AU - Mego, Michal
AU - Lee, Bang Ning
AU - Lodhi, Ashutosh
AU - Cristofanilli, Massimo
AU - Lucci, Anthony
AU - Reuben, James M.
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Purpose: The bone marrow microenvironment is considered a critical component in the dissemination and fate of cancer cells in the metastatic process. We explored the possible correlation between bone marrow mesenchymal stem cells (BM-MSC) and disseminated breast cancer-initiating cells (BCIC) in primary breast cancer patients. Results: The percentages of BCIC (Aldefluor +CD326+CD44+CD24-) correlated with the percentages of BM-MSC, either CD45-GD2+CD200 +CD271+ (Kedall's τ = 0.684, p = 0.004) or CD45 -GD2+CD271+ in the bone marrow (Kedall's τ = 0.464, p = 0.042). Experimental Design: Bone marrow mononuclear cells (BM-MNC) were collected at the time of primary surgery in 12 breast cancer patients. BM-MNC was immunophenotyped and BCIC was defined as epithelial cells (CD326 +CD45-) with a "stem-like" phenotype (CD44 +CD24low/-, ALDH activity). BM-MSC was defined as CD34-CD45- cells that co-expressed GD2, CD271 and/or CD200 within CD326-depleted BM-MNC. Conclusions: There was a positive correlation between mesenchymal stem cells expressing GD2 and CD271 and breast cancer-initiating cells in BM of patients with primary breast cancer.
AB - Purpose: The bone marrow microenvironment is considered a critical component in the dissemination and fate of cancer cells in the metastatic process. We explored the possible correlation between bone marrow mesenchymal stem cells (BM-MSC) and disseminated breast cancer-initiating cells (BCIC) in primary breast cancer patients. Results: The percentages of BCIC (Aldefluor +CD326+CD44+CD24-) correlated with the percentages of BM-MSC, either CD45-GD2+CD200 +CD271+ (Kedall's τ = 0.684, p = 0.004) or CD45 -GD2+CD271+ in the bone marrow (Kedall's τ = 0.464, p = 0.042). Experimental Design: Bone marrow mononuclear cells (BM-MNC) were collected at the time of primary surgery in 12 breast cancer patients. BM-MNC was immunophenotyped and BCIC was defined as epithelial cells (CD326 +CD45-) with a "stem-like" phenotype (CD44 +CD24low/-, ALDH activity). BM-MSC was defined as CD34-CD45- cells that co-expressed GD2, CD271 and/or CD200 within CD326-depleted BM-MNC. Conclusions: There was a positive correlation between mesenchymal stem cells expressing GD2 and CD271 and breast cancer-initiating cells in BM of patients with primary breast cancer.
KW - Bone marrow
KW - Cancer stem cells
KW - Cancer-initiating cells
KW - Mesenchymal stem cells
KW - Microenvironment
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U2 - 10.4161/cbt.11.9.15178
DO - 10.4161/cbt.11.9.15178
M3 - Article
C2 - 21358274
AN - SCOPUS:79955495408
VL - 11
SP - 812
EP - 815
JO - Cancer Biology and Therapy
JF - Cancer Biology and Therapy
SN - 1538-4047
IS - 9
ER -