Mesenchymal stem cells from preterm to term newborns undergo a significant switch from anaerobic glycolysis to the oxidative phosphorylation

Research output: Contribution to journalArticle

Abstract

We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs. These changes seem governed by the expression of CLUH, a cytosolic messenger RNA-binding protein involved in the mitochondria biogenesis and distribution inside the cell; in fact, CLUH silencing in term MSC determined a metabolic fingerprint similar to that of preterm MSC. Our study discloses novel information on the production of energy and mitochondrial organization and function, during the passage from fetal to adult life, providing useful information for the management of preterm birth.

Original languageEnglish
Pages (from-to)889-903
Number of pages15
JournalExperientia
Volume75
Issue number5
DOIs
Publication statusPublished - Mar 2018

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Oxidative Phosphorylation
Glycolysis
Stem cells
Mesenchymal Stromal Cells
Switches
Gestational Age
Reticulum
Mitochondria
Organizations
Mitochondrial Dynamics
Information Management
RNA-Binding Proteins
Umbilical Cord
Premature Birth
Dermatoglyphics
Energy Metabolism
Bone
Fusion reactions
Bone Marrow
Messenger RNA

Keywords

  • Anaerobiosis
  • Cells, Cultured
  • Energy Metabolism/physiology
  • Glycolysis/physiology
  • Humans
  • Infant, Newborn
  • Infant, Premature
  • Mesenchymal Stem Cells/cytology
  • Oxidative Phosphorylation
  • Premature Birth/metabolism
  • Term Birth/metabolism
  • Umbilical Cord/cytology

Cite this

@article{ef53569c93a84b46900d5070f3b5c472,
title = "Mesenchymal stem cells from preterm to term newborns undergo a significant switch from anaerobic glycolysis to the oxidative phosphorylation",
abstract = "We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs. These changes seem governed by the expression of CLUH, a cytosolic messenger RNA-binding protein involved in the mitochondria biogenesis and distribution inside the cell; in fact, CLUH silencing in term MSC determined a metabolic fingerprint similar to that of preterm MSC. Our study discloses novel information on the production of energy and mitochondrial organization and function, during the passage from fetal to adult life, providing useful information for the management of preterm birth.",
keywords = "Anaerobiosis, Cells, Cultured, Energy Metabolism/physiology, Glycolysis/physiology, Humans, Infant, Newborn, Infant, Premature, Mesenchymal Stem Cells/cytology, Oxidative Phosphorylation, Premature Birth/metabolism, Term Birth/metabolism, Umbilical Cord/cytology",
author = "Silvia Ravera and Marina Podest{\`a} and Federica Sabatini and Chiara Fresia and Marta Columbaro and Silvia Bruno and Ezio Fulcheri and Ramenghi, {Luca Antonio} and Francesco Frassoni",
year = "2018",
month = "3",
doi = "10.1007/s00018-017-2665-z",
language = "English",
volume = "75",
pages = "889--903",
journal = "Cellular and Molecular Life Sciences",
issn = "1420-682X",
publisher = "Birkhauser Verlag Basel",
number = "5",

}

TY - JOUR

T1 - Mesenchymal stem cells from preterm to term newborns undergo a significant switch from anaerobic glycolysis to the oxidative phosphorylation

AU - Ravera, Silvia

AU - Podestà, Marina

AU - Sabatini, Federica

AU - Fresia, Chiara

AU - Columbaro, Marta

AU - Bruno, Silvia

AU - Fulcheri, Ezio

AU - Ramenghi, Luca Antonio

AU - Frassoni, Francesco

PY - 2018/3

Y1 - 2018/3

N2 - We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs. These changes seem governed by the expression of CLUH, a cytosolic messenger RNA-binding protein involved in the mitochondria biogenesis and distribution inside the cell; in fact, CLUH silencing in term MSC determined a metabolic fingerprint similar to that of preterm MSC. Our study discloses novel information on the production of energy and mitochondrial organization and function, during the passage from fetal to adult life, providing useful information for the management of preterm birth.

AB - We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs. These changes seem governed by the expression of CLUH, a cytosolic messenger RNA-binding protein involved in the mitochondria biogenesis and distribution inside the cell; in fact, CLUH silencing in term MSC determined a metabolic fingerprint similar to that of preterm MSC. Our study discloses novel information on the production of energy and mitochondrial organization and function, during the passage from fetal to adult life, providing useful information for the management of preterm birth.

KW - Anaerobiosis

KW - Cells, Cultured

KW - Energy Metabolism/physiology

KW - Glycolysis/physiology

KW - Humans

KW - Infant, Newborn

KW - Infant, Premature

KW - Mesenchymal Stem Cells/cytology

KW - Oxidative Phosphorylation

KW - Premature Birth/metabolism

KW - Term Birth/metabolism

KW - Umbilical Cord/cytology

U2 - 10.1007/s00018-017-2665-z

DO - 10.1007/s00018-017-2665-z

M3 - Article

C2 - 28975370

VL - 75

SP - 889

EP - 903

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-682X

IS - 5

ER -