Mesenchymal stromal cells uptake and release paclitaxel without reducing its anticancer activity

Massimo Mariotti, Renato Colognato, Marco Rimoldi, Manuela Rizzetto, Francesca Sisto, Valentina Coccè, Arianna Bonomi, Eugenio Parati, Giulio Alessandri, Renzo Bagnati, Augusto Pessina

Research output: Contribution to journalArticle

Abstract

To improve the drug delivery efficiency on target cells, many strategies have been developed including Mesenchymal Stromal Cells (MSCs) approaches. In a previous study, we found that bone-marrow-derived MSCs (BM-MSCs) were able to incorporate and release the anti-tumor and anti-angiogenic drug, Paclitaxel (PTX). In this study, we evaluated the stability of PTX in standard cell culture conditions by analyzing the metabolites produced by MSCs after their incorporation of the drug. We are able to show that MSCs do not release either 3-OH-PTX or 6-OH-PTX metabolites (having a lower anticancer activity) but release an active PTX molecule together with the isomer 7-Epitaxol, is known to maintain the whole biological activity. This confirms that the simple procedure of MSCs priming with a drug (without any genetic cell manipulation), in our case PTX, does not modify the activity of the molecule and provides a new biological-device to carry and deliver PTX in tumor sites, by contributing to improve drug efficacy and target selectivity in cancer therapy.

Original languageEnglish
Pages (from-to)400-405
Number of pages6
JournalAnti-Cancer Agents in Medicinal Chemistry
Volume15
Issue number3
Publication statusPublished - 2015

Keywords

  • Anti-tumor activity
  • Cancer
  • Drug delivery
  • Mesenchymal stromal cell
  • Paclitaxel

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Pharmacology
  • Medicine(all)

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  • Cite this

    Mariotti, M., Colognato, R., Rimoldi, M., Rizzetto, M., Sisto, F., Coccè, V., Bonomi, A., Parati, E., Alessandri, G., Bagnati, R., & Pessina, A. (2015). Mesenchymal stromal cells uptake and release paclitaxel without reducing its anticancer activity. Anti-Cancer Agents in Medicinal Chemistry, 15(3), 400-405.