Mesodermal expression of Tbx1 is necessary and sufficient for pharyngeal arch and cardiac outflow tract development

Zhen Zhang, Tuong Huynh, Antonio Baldini

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The development of the segmented pharyngeal apparatus involves complex interaction of tissues derived from all three germ layers. The role of mesoderm is the least studied, perhaps because of its apparent lack of anatomical boundaries and positionally restricted gene expression. Here, we report that the mesoderm-specific deletion of Tbx1, a T-box transcription factor, caused severe pharyngeal patterning and cardiovascular defects, while mesoderm-specific restoration of Tbx1 expression in a mutant background corrected most of those defects in the mouse. We show that some organs, e.g. the thymus, require Tbx1 expression in the mesoderm and in the epithelia. In addition, these experiments revealed that different pharyngeal arches require Tbx1 in different tissues. Finally, we show that Tbx1 in the mesoderm is required to sustain cell proliferation. Thus, the mesodermal transcription program is not only crucial for cardiovascular development, but is also key in the development and patterning of pharyngeal endoderm.

Original languageEnglish
Pages (from-to)3587-3595
Number of pages9
JournalDevelopment
Volume133
Issue number18
DOIs
Publication statusPublished - Sep 2006

Fingerprint

Branchial Region
Mesoderm
Germ Layers
Endoderm
Thymus Gland
Transcription Factors
Epithelium
Cell Proliferation
Gene Expression

Keywords

  • 22q11DS
  • Anterior heart field
  • Cardiac outflow tract
  • DiGeorge syndrome
  • Fgf8
  • Mesoderm
  • Mouse
  • Pharyngeal development
  • Tbx1
  • Thymus

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Mesodermal expression of Tbx1 is necessary and sufficient for pharyngeal arch and cardiac outflow tract development. / Zhang, Zhen; Huynh, Tuong; Baldini, Antonio.

In: Development, Vol. 133, No. 18, 09.2006, p. 3587-3595.

Research output: Contribution to journalArticle

Zhang, Zhen ; Huynh, Tuong ; Baldini, Antonio. / Mesodermal expression of Tbx1 is necessary and sufficient for pharyngeal arch and cardiac outflow tract development. In: Development. 2006 ; Vol. 133, No. 18. pp. 3587-3595.
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