Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes

Francesca Salamanna, A. Frizziero, S. Pagani, G. Giavaresi, D. Curzi, E. Falcieri, M. Marini, P. M. Abruzzo, L. Martini, M. Fini

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to investigate tenocyte mechanobiology after sudden-detraining and to examine the hypothesis that repeated peri-patellar injections of hyaluronic acid (HA) on detrained patellar tendon (PT) may reduce and limit detrained-associated damage in tenoctyes. Twenty-four male Sprague-Dawley rats were divided into three groups: Untrained, Trained and Detrained. In the Detrained rats, the left tendon was untreated while the right tendon received repeated peri-patellar injections of either HA or saline (NaCl). Tenocyte morphology, metabolism and synthesis of C-terminal-propeptide of type I collagen, collagen-III, fibronectin, aggrecan, tenascin-c, interleukin-1β, matrix-metalloproteinase-1 and-3 were evaluated after 1, 3, 7 and 10 days of culture. Transmission-electronic-microscopy showed a significant increase in mitochondria and rough endoplasmic reticulum in cultured tenocytes from Detrained-HA with respect to those from Detrained-NaCl. Additionally, Detrained-HA cultures showed a significantly higher proliferation rate and viability, and increased synthesis of C-terminal-Propeptide of type I collagen, fibronectin, aggrecan, tenascin-c and matrix-metalloproteinase-3 with respect to Detrained-NaCl ones, whereas synthesis of matrix-metalloproteinase-1 and interleukin-1β was decreased. Our study demonstrates that discontinuing training activity in the short-term alters tenocyte synthetic and metabolic activity and that repeated peri-patellar infiltrations of HA during detraining allow the maintenance of tenocyte anabolic activity.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalConnective Tissue Research
Volume56
Issue number1
DOIs
Publication statusPublished - Feb 1 2015

Fingerprint

Hyaluronic Acid
Tendons
Injections
Tenascin
Aggrecans
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Collagen Type I
Interleukin-1
Fibronectins
Rats
Biophysics
Patellar Ligament
Mitochondria
Rough Endoplasmic Reticulum
Infiltration
Metabolism
Sprague Dawley Rats
Microscopy
Microscopic examination

Keywords

  • Detraining
  • Hyaluronic acid
  • Patellar tendon
  • Rats
  • Tenocytes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology
  • Medicine(all)

Cite this

Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes. / Salamanna, Francesca; Frizziero, A.; Pagani, S.; Giavaresi, G.; Curzi, D.; Falcieri, E.; Marini, M.; Abruzzo, P. M.; Martini, L.; Fini, M.

In: Connective Tissue Research, Vol. 56, No. 1, 01.02.2015, p. 35-43.

Research output: Contribution to journalArticle

Salamanna, Francesca ; Frizziero, A. ; Pagani, S. ; Giavaresi, G. ; Curzi, D. ; Falcieri, E. ; Marini, M. ; Abruzzo, P. M. ; Martini, L. ; Fini, M. / Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes. In: Connective Tissue Research. 2015 ; Vol. 56, No. 1. pp. 35-43.
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AU - Curzi, D.

AU - Falcieri, E.

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AB - The purpose of this study was to investigate tenocyte mechanobiology after sudden-detraining and to examine the hypothesis that repeated peri-patellar injections of hyaluronic acid (HA) on detrained patellar tendon (PT) may reduce and limit detrained-associated damage in tenoctyes. Twenty-four male Sprague-Dawley rats were divided into three groups: Untrained, Trained and Detrained. In the Detrained rats, the left tendon was untreated while the right tendon received repeated peri-patellar injections of either HA or saline (NaCl). Tenocyte morphology, metabolism and synthesis of C-terminal-propeptide of type I collagen, collagen-III, fibronectin, aggrecan, tenascin-c, interleukin-1β, matrix-metalloproteinase-1 and-3 were evaluated after 1, 3, 7 and 10 days of culture. Transmission-electronic-microscopy showed a significant increase in mitochondria and rough endoplasmic reticulum in cultured tenocytes from Detrained-HA with respect to those from Detrained-NaCl. Additionally, Detrained-HA cultures showed a significantly higher proliferation rate and viability, and increased synthesis of C-terminal-Propeptide of type I collagen, fibronectin, aggrecan, tenascin-c and matrix-metalloproteinase-3 with respect to Detrained-NaCl ones, whereas synthesis of matrix-metalloproteinase-1 and interleukin-1β was decreased. Our study demonstrates that discontinuing training activity in the short-term alters tenocyte synthetic and metabolic activity and that repeated peri-patellar infiltrations of HA during detraining allow the maintenance of tenocyte anabolic activity.

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